PDF DatasheetChicken MDH1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant MDH1 based upon sequence from Chicken
Host: QP12665 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of MDH1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Definition of Unit (for specific activity): One unit is defined as 1 umol of NAD+ production per minute under the assay conditions (25°C, pH 7.5).
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Malate dehydrogenase in sterile 18MΩ.cm H2O.
Bioactivity Data:
Specific Activity: Specific Activity Greater than 710U/mg protein.
Amino Acid Sequence: MGEPIRVLVT GAAGQIAYSL LYSIAKGDVF GKEQPLVLVL LDITPMMTVL EGVVMELQDC ALPLLREVIP TDKEEVAFKD LDIAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA KKTVKVVVVG NPANTNCLIA SKSAPSIPKE NFSCLTRLDH NRAKSQIALK LGVTSNDVKN VIIWGNHSST QYPDVNHAKV NVKGKEVGVY EAIKDDSWLK GDFILTVQQR GAAVIKARKL SSAMSAAKAI CDHVRDIWFG TPAGEFVSMG VISDGNSYGV PEDLLYSFPV VIKDKTWKFV EGLPINDFSR EKMDLTAKEL TEEKETAVEF LSSA
Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Each mg of protein contains 0.59 mg NaPO4.
Storage Conditions: Lyophilized Malate dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MDH1 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile lyophilized powder.
| Recombinant Chicken MDH1 General Information | |
|---|---|
| Alternate Names | |
| Malate dehydrogenase cytoplasmic, EC 1.1. 1.37, Cytosolic malate dehydrogenase, MDHA, MOR2, MDH-s, MGC:1375, MDH1. | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q5ZME2 |
| General Description of Recombinant Chicken MDH1. | |
| The DNA encoding Malate (Malic) Dehydrogenase is cloned from cDNA library of chicken heart. The MDH1 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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