PDF Datasheetother MIF T. Vaginalis Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant MIF T. Vaginalis based upon sequence from other
Host: QP12697 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of MIF T. Vaginalis was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to add 200µl deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. MIF T. Vaginalis is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: MPALVIKTNA KFTEEEKSKA TEELGNIVSK VLGKPISYVM VTLEDGVAVR FGGSDEKAAF MSLMSIGGLN RAVNKRASAA LTKWFTDHGF QGDRIYIVFN PKSAEDWGFN GDTFAHHHHH H
Purity: Greater than 95.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: MIF T. Vaginalis filtered (0.4 µm) and lyophilized from 0.5 mg/ml solution in 20 mM Tris buffer and 50 mM NaCl, pH 7.5.
Storage Conditions: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
| Recombinant other MIF T. Vaginalis General Information | |
|---|---|
| Alternate Names | |
| Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor), Phenylpyruvate Tautomerase, L-Dopachrome Tautomerase, L-Dopachrome Isomerase, GLIF, MMIF, GIF, Macrophage Migration Inhibitory Factor, Glycosylation-Inhibiting Factor, EC 5.3. 3.12, EC 5.3. 2.1, Macrophage migration inhibitory factor. | |
| Molecular Weight | |
| 13.4 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | A2DXT4 |
| General Description of Recombinant other MIF T. Vaginalis. | |
| MIF T. Vaginalis Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain (a. a 1-115) containing 121 amino acids including a 6 a. a C-terminal His tag. The total molecular mass is 13.4kDa (calculated). | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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