PDF DatasheetHuman NPPA Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant NPPA based upon sequence from Human
Host: QP12873 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of NPPA was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to add 200µl deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. NPPA is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: MKHHHHHHNP MYNAVSNADL MDFKNLLDHL EEKMPLEDEV VPPQVLSEPN EEAGAALSPL PEVPPWTGEV SPAQRDGGAL GRGPWDSSDR SALLKSKLRA LLTAPR
Purity: Greater than 95.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: NPPA filtered (0.4 µm) and lyophilized from 0.5 mg/ml in 20 mM Tris buffer, 50 mM NaCl and 5% w/v trehalosa, pH 7.5.
Storage Conditions: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human NPPA General Information | |
|---|---|
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| Alternate Names | |
| Natriuretic peptides A, CDD-ANF, Cardiodilatin, CDD, Cardiodilatin-related peptide, CDP, N-terminal proatrial natriuretic peptide, ANP, PND. | |
| Molecular Weight | |
| 11.7 kDa | |
| Chromosomal Location | |
| p36.22 on chromosome 1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | NPPA |
| Entrez Gene ID | 4878 |
| UniProt ID(s) | P01160 |
| COSMIC ID Link(s) | NPPA |
| KEGG Gene ID(s) | hsa:4878 |
| General Description of Recombinant Human NPPA. | |
| Human NPPA produced in E. Coli is a single, non-glycosylated, polypeptide chain (a. a 26-123) containing 106 amino acids including an 8 a. a N-terminal His tag. The total molecular mass is 11.7kDa (calculated). | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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