PDF DatasheetHuman PEDF Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PEDF based upon sequence from Human
Host: QP13005 protein expressed in HEK 293, E. Coli.
Available Tags: Flag Tag, His, Untagged
Protein Construction: A cDNA sequence encoding the sequence of PEDF was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recomnded to add deionized water to a working concentration of 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: QNPASPPEEG SPDPDSTGAL VEEEDPFFKV PVNKLAAAVS NFGYDLYRVR SSTSPTTNVL LSPLSVATAL SALSLGAEQR TESIIHRALY YDLISSPDIH GTYKELLDTV TAPQKNLKSA SRIVFEKKLR IKSSFVAPLE KSYGTRPRVL TGNPRLDLQE INNWVQAQMK GKLARSTKEI PDEISILLLG VAHFKGQWVT KFDSRKTSLE DFYLDEERTV RVPMMSDPKA VLRYGLDSDL SCKIAQLPLT GSMSIIFFLP LKVTQNLTLI EESLTSEFIH DIDRELKTVQ AVLTVPKLKL SYEGEVTKSL QEMKLQSLFD SPDFSKITGK PIKLTQVEHR AGFEWNEDGA GTTPSPGLQP AHLTFPLDYH LNQPFIFVLR DTDTGALLFI GKILDPRGPA AADYKDDDDK
Purity: Greater than 95% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The filtered (0.4µm) concentrated (0.5 mg/ml) protein solution was lyophilized with 20mM Tris & 20mM NaCl pH 7.5.
Storage Conditions: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human PEDF General Information | |
|---|---|
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| Alternate Names | |
| Pigment epithelium-derived factor, PEDF, Serpin-F1, SerpinF1, EPC-1, EPC1, PIG35. | |
| Molecular Weight | |
| 45.6 kDa | |
| Chromosomal Location | |
| p13.3 on chromosome 17 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SERPINF1 |
| Entrez Gene ID | 5176 |
| UniProt ID(s) | P36955 |
| COSMIC ID Link(s) | SERPINF1 |
| KEGG Gene ID(s) | hsa:5176 |
| General Description of Recombinant Human PEDF. | |
| Human PEDF produced in HEK cells is a single, glycosylated, polypeptide chain containing a total of 410 amino acids, having a molecular mass of 45.6 kDa and fused to an 11 aa FLAG tag at C-Terminus. The Human PEDF is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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