PDF DatasheetHuman pGH 22kDa Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant pGH 22kDa based upon sequence from Human
Host: QP13030 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of pGH 22kDa was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Placental HGH in 0.4% NaHCO3or water adjusted to pH 9, not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Amino Acid Sequence: MAAGSRTSLL LAFGLLCLSW LQEGSAFPTI PLSRLFDNAM LRARRLYQLA YDTYQEFEEA YILKEQKYSF LQNPQTSLCF SESIPTPSNR VKTQQKSNLE LLRISLLLIQ SWLEPVQLLR SVFANSLVYG ASDSNVYRHL KDLEEGIQTL MWRLEDGSPR TGQIFNQSYS KFDTKSHNDD ALLKNYGLLY CFRKDMDKVE TFLRIVQCRS VEGSCGF
Purity: Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Concentration of Human pGH 22kDa Protein: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.18 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GH-22K-placental as a Reference Standard.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045mM NaHCO3 previously adjusted pH 8-9.
Storage Conditions: Lyophilized GH placental although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution and filter sterilization HGH placental can be stored at 4°C for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human pGH 22kDa General Information | |
|---|---|
| Alternate Names | |
| GHL, GHV, GH-V, hGH-V, PGH. | |
| Molecular Weight | |
| 22.4 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P01242 |
| General Description of Recombinant Human pGH 22kDa. | |
| Human Placental HGH 22kDa Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 192 amino acids and having a molecular mass of 22367 Dalton. Predicted pI=7.80. Placental Growth Hormone has diminished lactogenic (prolactin receptor mediated) activity characteristic to pituitary GHs. GH Placental is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Selected References
Protein Quantification by Isotope Dilution Mass Spectrometry of Proteolytic Fragments: Cleavage Rate and Accuracy.Publication:Anal. Chem., 2008, 80 (11), pp 41544160 DOI: 10.1021/ac7024738Publication Date (Web): April 30, 2008Copyright © 2008 American Chemical Society.Link:http://pubs.acs.org/doi/full/10.1021/ac7024738
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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