PDF DatasheetHuman PGLYRP1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PGLYRP1 based upon sequence from Human
Host: QP13033 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of PGLYRP1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to add 200µl of 0.1M Acetate buffer pH 4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely at 37°C. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited. PGLYRP1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: MKHHHHHHASQETEDPACCS PIVPRNEWKA LASECAQHLS LPLRYVVVSH TAGSSCNTPA SCQQQARNVQ HYHMKTLGWC DVGYNFLIGE DGLVYEGRGW NFTGAHSGHL WNPMSIGISF MGNYMDRVPT PQAIRAAQGL LACGVAQGAL RSNYVLKGHR DVQRTLSPGN QLYHLIQNWP HYRSP
Purity: Greater than 90.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: PGLYRP1 filtered (0.4 µm) and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH 4.0.
Storage Conditions: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human PGLYRP1 General Information | |
|---|---|
| Alternate Names | |
| Peptidoglycan recognition protein 1, Peptidoglycan recognition protein short, PGRP-S, PGLYRP1, PGLYRP, PGRP, TNFSF3L, TAG7, PGRPS. | |
| Molecular Weight | |
| 20.68 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | O75594 |
| General Description of Recombinant Human PGLYRP1. | |
| Human PGLYRP1 produced in E. Coli is a single, non-glycosylated, polypeptide chain (a. a 22-196) containing 185 amino acids including a 10 a. a N-terminal His tag. The total molecular mass is 20.68kDa (calculated). | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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