PDF DatasheetHuman PKAR-I alpha Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PKAR-I alpha based upon sequence from Human
Host: QP13062 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of PKAR-I alpha was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Other Applications: The cAMP-dependent protein kinase, regulatory subunit RIa reversibly inhibits the catalytic subunit Ca of the cAMP-dependent protein kinase PKA. The inhibition of the catalytic subunit can be reversed by the addition of the second messenger cAMP.
Bioactivity Data: Untested
Monomer or Dimer: Dimer
Amino Acid Sequence: MESGSTAASE EARSLRECEL YVQKHNIQAL LKDSIVQLCT ARPERPMAFL REYFERLEKE EAKQIQNLQK AGTRTDSRED EISPPPPNPV VKGRRRRGAI SAEVYTEEDA ASYVRKVIPK DYKTMAALAK AIEKNVLFSH LDDNERSDIF DAMFSVSFIA GETVIQQGDE GDNFYVIDQG ETDVYVNNEW ATSVGEGGSF GELALIYGTP RAATVKAKTN VKLWGIDRDS YRRILMGSTL RKRKMYEEFL SKVSILESLD KWERLTVADA LEPVQFEDGQ KIVVQGEPGD EFFIILEGSA AVLQRRSENE EFVEVGRLGP SDYFGEIALL MNRPRAATVV ARGPLKCVKL DRPRFERVLG PCSDILKRNI QQYNSFVSLS V
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: PKA regulatory subunit-I alpha is supplied at a concentration of 1.3 mg/ml in 20mM MOPS (pH 7.0), 150mM NaCl, 1mM 2-mercaptoethanol and 50% glycerol.
Storage Conditions: PKAR-Ia should be stored at 4°C if entire vial will be used within 2-4 weeks. For long term storage it is recommended to store at -20°C. Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human PKAR-I alpha General Information | |
|---|---|
| Alternate Names | |
| cAMP-dependent protein kinase type I-alpha regulatory subunit, Tissue-specific extinguisher 1, TSE1, PRKAR1A PKR1, PRKAR1, CAR, CNC, CNC1, PKR1, ADOHR, PPNAD1, ACRDYS1. | |
| Molecular Weight | |
| 43 kDa | |
| Chromosomal Location | |
| q24.2 on chromosome 17 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PRKAR1A |
| Entrez Gene ID | 5573 |
| UniProt ID(s) | P10644 |
| COSMIC ID Link(s) | PRKAR1A |
| KEGG Gene ID(s) | hsa:5573 |
| General Description of Recombinant Human PKAR-I alpha. | |
| Human PKA regulatory subunit I a is a dimeric 86kDa protein (the monomer is 381 aa 43kDa). PKAR-I alpha is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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