PDF DatasheetHuman PKC-a Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PKC-a based upon sequence from Human
Host: QP13064 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of PKC-a was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Definition of Unit (for specific activity): 7200- 9000 units/mg. One unit of PKC-a activity transfers 1 nmole of phosphate to a PKC substrate peptide (myelin basic protein 4-14) per minute at 30°C. Recombinant human active PKC-a also phosphorylates full length myelin basic protein, histone, MARKS, and GSK-3b. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions.
Additional Information: Dilute in 10mM HEPES (pH7.4), 0.01% CHAPS and 5mM DTT. *for further dilution you may use either the Storage Buffer or Dilution Buffer.
Bioactivity Data:
Specific Activity: 1570 nmole of phosphate transferred to Histone H1 substrate per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.5 to 2ug/ml.
Amino Acid Sequence: MADVYPGNDS TASQDVANRF ARKGALRQKN VHEVKDHKFI ARFFKQPTFC SHCTDFIWGF GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPDTDDPRS KHKFKIHTYG SPTFCDHCGS LLYGLIHQGM KCDTCDMNVH KQCVINVPSL CGMDHTEKRG RIYLKAEVAD EKLHVTVRDA KNLIPMDPNG LSDPYVKLKL IPDPKNESKQ KTKTIRSTLN PQWNESFTFK LKPSDKDRRL SVEIWDWDRT TRNDFMGSLS FGVSELMKMP ASGWYKLLNQ EEGEYYNVPI PEGDEEGNME LRQKFEKAKL GPAGNKVISP SEDRKQPSNN LDRVKLTDFN FLMVLGKGSF GKVMLADRKG TEELYAIKIL KKDVVIQDDD VECTMVEKRV LALLDKPPFL TQLHSCFQTV DRLYFVMEYV NGGDLMYHIQ QVGKFKEPQA VFYAAEISIG LFFLHKRGII YRDLKLDNVM LDSEGHIKIA DFGMCKEHMM DGVTTRTFCG TPDYIAPEII AYQPYGKSVD WWAYGVLLYE MLAGQPPFDG EDEDELFQSI MEHNVSYPKS LSKEAVSICK GLMTKHPAKR LGCGPEGERD VREHAFFRGI DWEKLENREI QPPFKPKVCG KGAENFDKFF TRGQPVLTPP DQLVIANIDQ SDFEGFSYVN PQFVHPILQS AV
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: PKC-a is supplied in 20mM HEPES pH 7.5, 250mM NaCl, 50% glycerol, 2mM EDTA, 2mM EGTA, 0.05%Triton X-100 and 5mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Colorless Liquid
| Recombinant Human PKC-a General Information | |
|---|---|
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| Alternate Names | |
| Protein kinase C alpha type, EC 2.7. 11.13, PKC-alpha, PKC-A, PRKCA, AAG6, PKCA, PRKACA, MGC129900, MGC129901. | |
| Molecular Weight | |
| 76.7 kDa | |
| Chromosomal Location | |
| q24.2 on chromosome 17 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PRKCA |
| Entrez Gene ID | 5578 |
| UniProt ID(s) | P17252 |
| COSMIC ID Link(s) | PRKCA |
| KEGG Gene ID(s) | hsa:5578 |
| General Description of Recombinant Human PKC-a. | |
| Human Protein Kinase C alpha produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-672 and having a molecular mass of 76.7 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag. PKC-a is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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