PDF DatasheetHuman PRKCE Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PRKCE based upon sequence from Human
Host: QP13145 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of PRKCE was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Definition of Unit (for specific activity): 3250 units/mg. One unit of PRKCE activity transfers 1 nmole of phosphate to a PRKCE substrate peptide per minute at 30°C. Recombinant human active PRKCE also phosphorylates full length myelin basic protein, MARKS, GAP-34, and raf-1 kinase. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application.
Bioactivity Data: Untested
Amino Acid Sequence: MVVFNGLLKI KICEAVSLKP TAWSLRHAVG PRPQTFLLDP YIALNVDDSR IGQTATKQKT NSPAWHDEFV TDVCNGRKIE LAVFHDAPIG YDDFVANCTI QFEELLQNGS RHFEDWIDLE PEGRVYVIID LSGSSGEAPK DNEERVFRER MRPRKRQGAV RRRVHQVNGH KFMATYLRQP TYCSHCRDFI WGVIGKQGYQ CQVCTCVVHK RCHELIITKC AGLKKQETPD QVGSQRFSVN MPHKFGIHNY KVPTFCDHCG SLLWGLLRQG LQCKVCKMNV HRRCETNVAP NCGVDARGIA KVLADLGVTP DKITNSGQRR KKLIAGAESP QPASGSSPSE EDRSKSAPTS PCDQEIKELE NNIRKALSFD NRGEEHRAAS SPDGQLMSPG ENGEVRQGQA KRLGLDEFNF IKVLGKGSFG KVMLAELKGK DEVYAVKVLK KDVILQDDDV DCTMTEKRIL ALARKHPYLT QLYCCFQTKD RLFFVMEYVN GGDLMFQIQR SRKFDEPRSR FYAAEVTSAL MFLHQHGVIY RDLKLDNILL DAEGHCKLAD FGMCKEGILN GVTTTTFCGT PDYIAPEILQ ELEYGPSVDW WALGVLMYEM MAGQPPFEAD NEDDLFESIL HDDVLYPVWL SKEAVSILKA FMTKNPHKRL GCVASQNGED AIKQHPFFKE IDWVLLEQKK IKPPFKPRIK TKRDVNNFDQ DFTREEPVLT LVDEAIVKQI NQEEFKGFSY FGEDLMP
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: PRKCE is supplied in 20mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF, 1mM benzamidine and 0.03% Brij-35.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human PRKCE General Information | |
|---|---|
| Alternate Names | |
| Protein kinase C epsilon type, EC 2.7. 11.13, nPKC-epsilon, PRKCE, PKCE, MGC125656, MGC125657. | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q02156 |
| General Description of Recombinant Human PRKCE. | |
| Human PRKCE produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-737 and having a molecular mass of 87 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag. PRKCE is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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