PDF DatasheetHuman Proguanylin Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Proguanylin based upon sequence from Human
Host: QP13157 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of Proguanylin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Proguanylin is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: MKHHHHHHAS VTVQDGNFSF SLESVKKLKD LQEPQEPRVG KLRNFAPIPG EPVVPILCSN PNFPEELKPL CKEPNAQEIL QRLEEIAEDP GTCEICAYAA CTGC
Purity: Greater than 90.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Proguanylin filtered (0.4 µm) and lyophilized in 0.5 mg/ml in deionized H20.
Storage Conditions: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human Proguanylin General Information | |
|---|---|
| Alternate Names | |
| Guanylin Precursor, Guanylate cyclase activator 2A, Guanylate cyclase-activating protein 1, Gap-IGUCA2, STARA, GUANYLIN. | |
| Molecular Weight | |
| 11.5 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q02747 |
| General Description of Recombinant Human Proguanylin. | |
| Human Proguanylin produced in E. coli is a single, non-glycosylated polypeptide chain (a. a 22-115) containing 104 amino acids including a 10 a. a N-terminal His tag. The total molecular mass is 11.5kDa (calculated). | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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