PDF DatasheetHuman SFRP4 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant SFRP4 based upon sequence from Human
Host: QP13478 protein expressed in HEK 293.
Available Tags: Flag Tag
Protein Construction: A cDNA sequence encoding the sequence of SFRP4 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to add 200µl deionized water to a working concentration of 0.5 mg/ml and let the lyophilized pellet dissolve completely. SFRP4 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: PGDYKDDDDK PAGVRGAPCE AVRIPMCRHM PWNITRMPNH LHHSTQENAI LAIEQYEELV DVNCSAVLRF FLCAMYAPIC TLEFLHDPIK PCKSVCQRAR DDCEPLMKMY NHSWPESLAC DELPVYDRGV CISPEAIVTD LPEDVKWIDI TPDMMVQERP LDVDCKRLSP DRCKCKKVKP TLATYLSKNY SYVIHAKIKA VQRSGCNEVT TVVDVKEIFK SSSPIPRTQV PLITNSSCQC PHILPHQDVL IMCYEWRSRM MLLENCLVEK WRDQLSKRSI QWEERLQEQR RTVQDKKKTA GRTSRSNPPK PKGKPPAPKP ASPKKNIKTR SAQKRTNPKR V
Purity: Greater than 90.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Filtered (0.4µm) and lyophilized from 0.5 mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4.
Storage Conditions: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human SFRP4 General Information | |
|---|---|
| Alternate Names | |
| Secreted frizzled-related protein 4, sFRP-4, Frizzled protein, human endometrium, FrpHE, SFRP4, FRPHE, FRP-4. | |
| Molecular Weight | |
| 39 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q6FHJ7 |
| General Description of Recombinant Human SFRP4. | |
| Human SFRP4 produced in HEK cells is a single, glycosylated, polypeptide chain (a. a 19-346) containing a total of 341 amino acids, having a molecular mass of 39kDa (calculated), though it migrates at approximately 55kDa on SDS PAGE, the SFRP4 is fused to a 5 a. a N-terminal linker and an 8 a. a Flag tag at N-Terminus. The Human SFRP4 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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