PDF DatasheetHuman SOST Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant SOST based upon sequence from Human
Host: QP13561 protein expressed in E. Coli, HEK 293.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of SOST was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to add 0.1M Acetate buffer pH 4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited. SOST is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: MKHHHHHHASQGWQAFKNDA TEIIPELGEY PEPPPELENN KTMNRAENGG RPPHHPFETK DVSEYSCREL HFTRYVTDGP CRSAKPVTEL VCSGQCGPAR LLPNAIGRGK WWRPSGPDFR CIPDRYRAQR VQLLCPGGEA PRARKVRLVA SCKCKRLTRF HNQSELKDFG TEAARPQKGR KPRPRARSAK ANQAELENAY
Purity: Greater than 90.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: SOST filtered (0.4 µm) and lyophilized from 0.5 mg/ml in 0.03M Acetate buffer pH 4.0.
Storage Conditions: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human SOST General Information | |
|---|---|
| Alternate Names | |
| Sclerostin, SOST, CDD, VBCH. | |
| Molecular Weight | |
| 22.8 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9BQB4 |
| General Description of Recombinant Human SOST. | |
| Human SOST produced in E. Coli is a single, non-glycosylated, polypeptide chain (a. a 24-213) containing 200 amino acids including a 10 a. a N-terminal His tag. The total molecular mass is 22.8kDa (calculated). | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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