PDF DatasheetHuman STC 2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant STC 2 based upon sequence from Human
Host: QP13613 protein expressed in HEK 293, E. Coli.
Available Tags: Untagged, His
Protein Construction: A cDNA sequence encoding the sequence of STC 2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: Add deionized water to a working concentration approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: TDATNPPEGP QDRSSQQKGR LSLQNTAEIQ HCLVNAGDVG CGVFECFENN SCEIRGLHGI CMTFLHNAGKFDAQGKSFIK DALKCKAHAL RHRFGCISRK CPAIREMVSQ LQRECYLKHD LCAAAQENTR VIVEMIHFKDLLLHEPYVDL VNLLLTCGEE VKEAITHSVQ VQCEQNWGSL CSILSFCTSA IQKPPTAPPE RQPQVDRTKLSRAHHGEAGH HLPEPSSRET GRGAKGERGS KSHPNAHARG RVGGLGAQGP SGSSEWEDEQ SEYSDIRRAAADYKDDDDK
Purity: Greater than 90.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Filtered (0.4µm) and lyophilized in 0.5 mg/ml in 20mM Tris buffer, 20mM NaCl, pH 7.5.
Storage Conditions: Lyophilized STC-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Stanniocalcin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: White lyophilized (freeze-dried) powder.
| Recombinant Human STC 2 General Information | |
|---|---|
| Alternate Names | |
| Stanniocalcin-2, STC, STC2, STCRP, STC-2, Stanniocalcin-related protein, STC-related protein. | |
| Molecular Weight | |
| 31.9 kDa | |
| Chromosomal Location | |
| q35.2 on chromosome 5 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | STC2 |
| Entrez Gene ID | 8614 |
| UniProt ID(s) | O76061 |
| COSMIC ID Link(s) | STC2 |
| KEGG Gene ID(s) | hsa:8614 |
| General Description of Recombinant Human STC 2. | |
| Human Stanniocalcin-2 Human Recombinant produced in HEK 293 cell line is a single, glycosylated, polypeptide chain containing 289 amino acids and having a total molecular mass of 31.9kDa (calculated). The Stanniocalcin contains four extra residues which were used as a spacer and 8 residues form the C-Terminal Flag- tag. Stanniocalcin is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. The amino acid sequence of the Stanniocalcin-2 is 100% homologous to the amino acid sequence AA 25-302 of the human mature Human Stanniocalcin-2. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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