PDF DatasheetStreptavidin Protein Product Attributes
Product Type: Native or Recombinant Protein
Streptavidin based upon sequence from other
Host: QP13625 protein expressed in Native Protein, E. Coli.
Available Tags: Untagged (native), His (E. coli)
Protein Construction: A cDNA sequence encoding the sequence of Streptavidin was constructed and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: Reconstitute in ultra-pure water. Gives a clear solution at 5 mg/ml in 0.1M NaCl.
Bioactivity Data:>15 U/mg (one unit binds 1 ug D-biotin at pH 8.9, less than 10^-3 U/mg protein (Azcoll, 25C, 24h, pH 8.0)
Amino Acid Sequence: MRKIVVAAIA VSLTTVSITA SASADPSKDS KAQVSAAEAG ITGTWYNQLG STFIVTAGAD GALTGTYESA VGNAESRYVL TGRYDSAPAT DGSGTALGWT VAWKNNYRNA HSATTWSGQY VGGAEARINT QWLLTSGTTE ANAWKSTLVG HDTFTKVKPS AASIDAAKKA GVNNGNPLDA VQQ
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Lyophilized (1 mg/ml) in 50mM NaCl, pH 9.0.
Storage Conditions: Streptavidin although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. For longer storage in dissolved form add 1mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered lyophilized powder.
| Recombinant other Streptavidin General Information | |
|---|---|
| Molecular Weight | |
| 55 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P22629 |
| COSMIC ID Link(s) | – |
| General Description of Recombinant other Streptavidin. | |
| Streptavidin is a protein produced by Streptomyces avidinii and isolated by purification from fermentation broth. The pure, homogeneous protein shows predominantly one single band in SDS PAGE. Streptavidin consists of 4 identical subunits, each bearing an active binding site for biotin. Streptavidin has a molecular weight of 55kDa. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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