PDF Datasheetother TEV Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant TEV based upon sequence from other
Host: QP13725 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of TEV was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Definition of Unit (for specific activity): One unit is defined as the amount of enzyme needed to cleave 3 ug of fusion protein in 1 hour to 85 % completion at 30°C in a buffer containing 50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, and 1 mM DTT.
Application Notes: A number of variables can be changed to optimize the cleavage of any specific protein. The amount of rTEV, the temperature of the incubation, and the time needed for cleavage may be examined. If the protein of interest is heat-labile, then 4°C incubations are recommended. Reactions at 4°C will require longer incubation times and/or more rTEV.
Bioactivity Data: Untested
Amino Acid Sequence: GHIVWPDYAN ILKEVFGGAR MACVTSAHMA GANGSILKKA EETSRATMHK PVIFGEDYVT EADLPYTPLH LEVNAEMERM YYLGRRALTH GKRRKVSVNN KRNRRRKVAK TYVGRDSIVE KIVVPHTERK VDTTTAVKDT CNEVSTQLVH NSMPKRKKQK NFLPATSLSN VYAQTWSIVR KRHMQVEIIS KKSVRAKVKR FEGSVQLFAS VRHMYGERKR VDLRIDNWQQ KTLLDLAKRF KNERVDQSKL TFGSSGLVLR QGSYAPAHWY RHGMFIVRGR SDGMLVDARA KVTFAVCYSM THY
Purity: Greater than 90.0% as determined by analysis by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The rTEV contains 25mM Tris, 75mM NaCl, 5mM EDTA, 10mM GSH, 50% Glycerol.
Storage Conditions: rTEV although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
Physical Appearance: Sterile liquid formulation.
| Recombinant other TEV General Information | |
|---|---|
| Alternate Names | |
| rTEV, TEV, P1 protease. | |
| Molecular Weight | |
| 34.8 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q0GDU8 |
| COSMIC ID Link(s) | – |
| General Description of Recombinant other TEV. | |
| Recombinant TEV Protease (rTEV) is a site-specific protease purified from E. coli. The protease can be used for the removal of affinity tags from fusion proteins. The seven-amino-acid recognition site for rTEV is Glu-Asn-Leu-Tyr-Phe-Gln-Gly with cleavage occurring between Gln and Gly. The optimal temperature for cleavage is 30°C; however, the enzyme can be used at temperatures as low as 4°C. The rTEV contains His tag. The rTEV is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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