PDF DatasheetRat TFF3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant TFF3 based upon sequence from Rat
Host: QP13729 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of TFF3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to add 200µl deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: MQEFVGLSPS QCMVPANVRV DCGYPTVTSE QCNNRGCCFD SSIPNVPWCF KPLQETECT F DYKDDDDK
Purity: Greater than 95.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: TFF3 protein filtered (0.4µm) and lyophilized in 0.5 mg/ml in 20mM TRIS and 50mM NaCl, pH 7.5.
Storage Conditions: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Rat TFF3 General Information | |
|---|---|
| Alternate Names | |
| Trefoil factor 3, Intestinal trefoil factor, rITF, Polypeptide P1. B, rP1. B, Tff3, Itf. | |
| Molecular Weight | |
| 8.9 kDa | |
| Chromosomal Location | |
| p12 on chromosome 20 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Tff3 |
| Entrez Gene ID | 25563 |
| UniProt ID(s) | Q03191 |
| COSMIC ID Link(s) | Tff3 |
| KEGG Gene ID(s) | rno:25563 |
| General Description of Recombinant Rat TFF3. | |
| The Trefoil Factor-3 Rat was constructed as a recombinant protein with a 9 a. a C-terminal fusion of Flag-Tag (1 aa N-terminal+8 aa C-terminal). The TFF3 Rat produced in E. coli, is 7.7kDa protein containing a total of 68 amino acid residues. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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