PDF DatasheetMouse TIE1 Fc Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant TIE1 Fc based upon sequence from Mouse
Host: QP13751 protein expressed in CHO.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of TIE1 Fc was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized TIE-1 Fc Chimera in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Monomer or Dimer: Dimer
Amino Acid Sequence: MVWWGSSLLL PTLFLASHVG ASVDLTLLAN LRITDPQRFF LTCVSGEAGA GRSSDPPLLL EKDDRIVRTF PPGQPLYLAR NGSHQVTLRG FSKPSDLVGV FSCVGGAGAR RTRVLYVHNS PGAHLFPDKV THTVNKGDTA VLSAHVHKEK QTDVIWKNNG SYFNTLDWQE ADDGRFQLQL QNVQPPSSGI YSATYLEASP LGSAFFRLIV RGCGAGRWGP GCVKDCPGCL HGGVCHDHDG ECVCPPGFTG TRCEQACREG RFGQSCQEQC PGTAGCRGLT FCLPDPYGCS CGSGWRGSQC QEACAPGHFG ADCRLQCQCQ NGGTCDRFSG CVCPSGWHGV HCEKSDRIPQ ILSMATEVEF NIGTMPRINC AAAGNPFPVR GSMKLRKPDG TMLLSTKVIV EPDRTTAEFE VPSLTLGDSG FWECRVSTSG GQDSRRFKVN VKVPPVPLTA PRLLAKQSRQ LVVSPLVSFS GDGPISSVRL HYRPQDSTIA WSAIVVDPSE NVTLMNLKPK TGYNVRVQLS RPGEGGEGGW GPSALMTTDC PEPLLQPWLE SWHVEGPDRL RVSWSLPSVP LSGDGFLLRL WDGARGQERR ENISFPQART ALLTGLTPGT HYQLDVRLYH CTLLGPASPP AHVHLPPSGP PAPRHLHAQA LSDSEIQLMW QHPEAPSGPI SKYIVEIQVA GGSGDPQWMD VDRPEETSII VRGLNASTRY LFRVRASVQG LGDWSNTVEE ATLGNGLQSE GPVRESRAAE EGLDQQLVLA VVGSVSATCL TILAALLALV CIRRSCLHRR RTFTYQSGSG EETILQFSSG TLTLTRRPKP QPEPLSYPVL EWEDITFEDL IGEGNFGQVI RAMIKKDGLK MNAAIKMLKE YASENDHRDF AGELEVLCKL GHHPNIINLL GACENRGYLY IAIEYAPYGN LLDFLRKSRV LETDPAFARE HGTASTLSSR QLLRFASDAA NGMQYLSEKQ FIHRDLAARN VLVGENLASK IADFGLSRGE EVYVKKTMGR LPVRWMAIES LNYSVYTTKS DVWSFGVLLW EIVSLGGTPY CGMTCAELYE KLPQGYRMEQ PRNCDDEVYE LMRQCWRDRP YERPPFAQIA LQLGRMLEAR KAYVNMSLFE NFTYAGIDAT AEEA
Purity: Greater than 90.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: TIE-1 Fc Chimera was lyophilized from a concentrated (1 mg/ml) sterile solution containing 1x PBS.
Storage Conditions: Lyophilized sTIE-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TIE-1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Mouse TIE1 Fc General Information | |
|---|---|
| Alternate Names | |
| Tyrosine kinase with immunoglobulin-like and EGF-like domains 1, JTK14, TIE, TIE1. | |
| Molecular Weight | |
| 260 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q06806 |
| General Description of Recombinant Mouse TIE1 Fc. | |
| Mouse Soluble TIE-1 fused with the Fc part of human IgG1 produced in CHO cells is a glycosylated disulfide-linked homodimeric polypeptide chain containing amino acids 23-749 and having a total molecular mass of 260kDa. The Mouse TIE-1/Fc monomer has a calculated molecular mass of approximately 105kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 130kDa protein in SDS-PAGE under reducing conditions. The TIE1 Fc Chimera is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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