PDF DatasheetBovine TOP1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant TOP1 based upon sequence from Bovine
Host: QP13784 protein expressed in Native Protein.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of TOP1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Plate Coating Instructions: 0.5-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.
Other Applications: 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checkerboard analysis of positive/negative sera panels) and immunodot test with positive/negative sera panels.
Bioactivity Data: Untested
Amino Acid Sequence: DAKVLKDAKT KKVVESKKKA VQRLEEQLMK LEVQATDREE NKQIALGTSK LNYLDPRITV AWCKKWG
Purity: Greater than 70% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: TOP1 is supplied at a concentration of 1 mg/1ml in 16mM HEPES buffer pH 7.5, 400mM NaCl and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Colorless Liquid
| Recombinant Bovine TOP1 General Information | |
|---|---|
| Alternate Names | |
| DNA topoisomerase 1, EC 5.99.1. 2, DNA topoisomerase I, TOP1, Scl-70. | |
| Molecular Weight | |
| 7.7 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q2HPH0 |
| COSMIC ID Link(s) | – |
| General Description of Recombinant Bovine TOP1. | |
| Bovine DNA Topoisomerase-I purified from bovine tissues by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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