PDF DatasheetHuman TOP1 70kDa Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant TOP1 70kDa based upon sequence from Human
Host: QP13785 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of TOP1 70kDa was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Plate Coating Instructions: 0.5-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Other Applications: 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checkerboard analysis of positive/negative sera panels, including CDC international reference sera).
Bioactivity Data: Untested
Amino Acid Sequence: MSGDHLHNDS QIEADFRLND SHKHKDKHKD REHRHKEHKK EKDREKSKHS NSEHKDSEKK HKEKEKTKHK DGSSEKHKDK HKDRDKEKRK EEKVRASGDA KIKKEKENGF SSPPQIKDEP EDDGYFVPPK EDIKPLKRPR DEDDADYKPK KIKTEDTKKE KKRKLEEEED GKLKKPKNKD KDKKVPEPDN KKKKPKKEEE QKWKWWEEER YPEGIKWKFL EHKGPVFAPP YEPLPENVKF YYDGKVMKLS PKAEEVATFF AKMLDHEYTT KEIFRKNFFK DWRKEMTNEE KNIITNLSKC DFTQMSQYFK AQTEARKQMS KEEKLKIKEE NEKLLKEYGF CIMDNHKERI ANFKIEPPGL FRGRGNHPKM GMLKRRIMPE DIIINCSKDA KVPSPPPGHK WKEVRHDNKV TWLVSWTENI QGSIKYIMLN PSSRIKGEKD WQKYETARRL KKCVDKIRNQ YREDWKSKEM KVRQRAVALY FIDKLALRAG NEKEEGETAD TVGCCSLRVE HINLHPELDG QEYVVEFDFL GKDSIRYYNK VPVEKRVFKN LQLFMENKQP EDDLFDRLNT GILNKHLQDL MEGLTAKVFR TYNASITLQQ QLKELTAPDE NIPAKILSYN RANRAVAILC NHQRAPPKTF EKSMMNLQTK IDAKKEQLAD ARRDLKSAKA DAKVMKDAKT KKVVESKKKA VQRLEEQLMK LEVQATDREE NKQIALGTSK LNYLDPRITV AWCKKWGVPI EKIYNKTQRE KFAWAIDMAD EDYEF
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: TOP1 70kDa is supplied in 16mM HEPES buffer pH 8.0, 400mM NaCl, and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Colorless Liquid
| Recombinant Human TOP1 70kDa General Information | |
|---|---|
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| Alternate Names | |
| DNA topoisomerase 1, EC 5.99.1. 2, DNA topoisomerase I, TOP1, Scl-70. | |
| Molecular Weight | |
| 69.2 kDa | |
| Chromosomal Location | |
| q12 on chromosome 20 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TOP1 |
| Entrez Gene ID | 7150 |
| UniProt ID(s) | P11387 |
| COSMIC ID Link(s) | TOP1 |
| KEGG Gene ID(s) | hsa:7150 |
| General Description of Recombinant Human TOP1 70kDa. | |
| Recombinant TOP1 70kDa protein is an enzyme fragment having a molecular mass of 69,199 Dalton (pH 9.4). TOP1 70kDa protein is fused to a hexa-histidine purification tag. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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