PDF DatasheetHuman Urokinase Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Urokinase based upon sequence from Human
Host: QP13908 protein expressed in Native Protein.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Urokinase was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Urokinase in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data:
Specific Activity: 174,827IU/mg.
Purity: Greater than 94.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The Urokinase was lyophilized from a concentrated (1 mg/ml) solution containing phosphate buffer.
Storage Conditions: Lyophilized Urokinase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Urokinase should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Selected References
1. Transient ?-catenin stabilization modifies lineage output from human thymic CD34+CD1a progenitors. Publication:Published online before print December 1, 2009, doi: 10.1189/jlb.0509344 March 2010 Journal of Leukocyte Biology vol. 87 no. 3 405-414 .Link:http://www.jleukbio.org/content/87/3/405.full2. The human fibrinolytic system is a target for the staphylococcal metalloprotease aureolysinPublications: Biochem. J 410 (2008): 157-165.Link: http://www.biochemj.org/bj/410/bj4100157.htm3. Activation of human pro-urokinase by unrelated proteases secreted by Pseudomonas aeruginosa.Publication:Biochem. J 428 (2010): 473-482.Link:http://www.biochemj.org/bj/428/0473/4280473.pdf
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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