PDF DatasheetMouse VEGF (121 a. a. ) Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant VEGF (121 a. a. ) based upon sequence from Mouse
Host: QP13933 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of VEGF (121 a. a. ) was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized VEGF-121 in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Monomer or Dimer: Dimer
Amino Acid Sequence: MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKRPR R
Purity: Greater than 97.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The protein was lyophilized from a concentrated (1 mg/ml) solution with no additives.
Storage Conditions: Lyophilized VEGF-121 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF-121 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Mouse VEGF (121 a. a. ) General Information | |
|---|---|
| Alternate Names | |
| Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609. | |
| Molecular Weight | |
| 28.4 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q00731 |
| General Description of Recombinant Mouse VEGF (121 a. a. ). | |
| Mouse Vascular Endothelial Growth Factor-121 produced in E. Coli is a homodimer, non-glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of 28.4 kDa. Recombinant Mouse VEGF-121 is a truncated version of Murine VEGF-165. The VEGF-121 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Selected References
VEGF signaling mediates bladder neuroplasticity and inflammation in response to BCG.Publication:BMC Physiology 2011, 11:16 doi:10.1186/1472-6793-11-16Link:http://www.biomedcentral.com/1472-6793/11/16/
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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