PDF DatasheetHuman p16-INK4a, TAT Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant p16-INK4a, TAT based upon sequence from Human
Host: QP14024 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of p16-INK4a, TAT was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized p16-INK4a in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Amino Acid Sequence: EPAAGSSMEP SADWLATAAA RGRVEEVRAL LEAGALPNAP NSYGRRPIQV MMMGSARVAE LLLLHGAEPN CADPATLTRP VHDAAREGFL DTLVVLHRAG ARLDVRDAWG RLPVDLAEEL GHRDVARYLR AAAGGTRGSN HARIDAAEGP SDIPDGGYGR KKRRQRRR
Purity: Greater than 95.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Lyophilized from a 0.2µm filtered concentrated solution in 1xPBS, pH 7.4.
Storage Conditions: Lyophilized p16-INK4a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution p16-INK4a should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human p16-INK4a, TAT General Information | |
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| Alternate Names | |
| Cyclin-dependent kinase 4 inhibitor A, CDK4I, p16-INK4, p16-INK4a, p16INK4A, CDKN-2A, CDKN2, Multiple tumor suppressor 1, MTS1, CMM2, MLM, TP16, p16(INK4), p19. | |
| Molecular Weight | |
| 16.5 kDa | |
| Chromosomal Location | |
| p21.3 on chromosome 9 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CDKN2A |
| Entrez Gene ID | 1029 |
| UniProt ID(s) | P42771 |
| COSMIC ID Link(s) | CDKN2A |
| KEGG Gene ID(s) | hsa:1029 |
| General Description of Recombinant Human p16-INK4a, TAT. | |
| Human p16-INK4a is a single, non-glycosylated, polypeptide chain produced in E. coli, containing a total of 168 amino acids, which includes the 156 residues of full-length p16-INK4a and a 13-residue C-terminal TAT peptide (GGYGRKKRRQRRR), having a total MW of 18kDa. p16-INK4a is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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