Human, Baboon, Monkey, Cow, Pig, Sheep, Goat, Cat, Canine, Rabbit, Mouse, Rat, Guinea pig, and Chicken Anti-Actin, Smooth Muscle Antibody Product Attributes
Species: Human, Baboon, Monkey, Cow, Pig, Sheep, Goat, Cat, Canine, Rabbit, Mouse, Rat, Guinea pig, and Chicken
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Actin, Smooth Muscle Antibody Clone: 1A4; same as asm-1
Clone 1A4; same as asm-1 Host and Isotype: Mouse IgG2a kappa
Anti-Human, Baboon, Monkey, Cow, Pig, Sheep, Goat, Cat, Canine, Rabbit, Mouse, Rat, Guinea pig, and Chicken Actin, Smooth Muscle Positive Control Sample: Blood vessels in all tissues, smooth muscle or leiomyosarcoma
Cellular Localization of Antibody <1A4; same as asm-1 Staining: Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: N-Terminal decapeptide of alpha smooth muscle isoform of actin, conjugated to KLH.
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Actin, Smooth Muscle Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in Actin, Smooth Muscle antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in myoepithelial cells in the breast and smooth muscle cells in the smooth muscle. More moderate antibody staining intensity was present in myoepithelial cells in the breast and smooth muscle cells in the smooth muscle. Low, but measureable presence of Actin, Smooth Muscle could be seen in. We were unable to detect Actin, Smooth Muscle in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Actin, Smooth Muscle expression as measured by anti-Actin, Smooth Muscle antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | ++ | - | - | - | - |
ACTA2 Variability | ++ | + | ++ | ++ | + | + | + | + | ++ | ++ | ++ | + | + | + | + | +++ | + | + | ++ | + |
Limitations and Warranty
enQuire Bio's Actin, Smooth Muscle Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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