Human Anti-CD45 Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD45 Antibody Clone: 2D1
Clone 2D1 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5ul /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD45 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD45 / PTPRC antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of CD45 / PTPRC could be seen in. We were unable to detect CD45 / PTPRC in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of CD45 expression as measured by anti-CD45 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | - | - | - | - | - | - | - | - | - | +++ | - | - | - | - | - | - | - | - | - | - |
| PTPRC Variability | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
Selected References
Bradstock KF, Janossy G, Pizzolo G, Hoffbrand AV, McMichael A, Pilch JR, Milstein C, Beverley P and Bollum FJ. 1980. J. Natl. Cancer Inst. 65(1): 33-42.Schwinzer R. in Knapp W, Dorken B, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York. p. 628-634.Nieminen JK, Sipponen T, Färkkilä M and Vaarala O. 2014. Clin. Exp. Immunol. 177(1): 190-202. (Flow cytometry)Pi?tosa B, Wolska-Ku?nierz B, Pac M, Siewiera K, Ga?kowska E and Bernatowska E. 2010. Cytometry Part B. 78B: 372-381. (Flow cytometry)
Limitations and Warranty
enQuire Bio's Human Anti-CD45 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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