Human PBMCs were stained with 5 uL  (solid line) or 0.125 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.
Human PBMCs were stained with 5 uL  (solid line) or 0.125 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human PBMCs were stained with 5 uL  .Human PBMCs were stained with 5 uL  (solid line) or 0.25 ug Mouse IgG1 FITC isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human PBMCs were stained with 5 uL  .Human PBMCs were stained with 5 uL  .Human PBMCs were stained with 5 uL  (solid line) or 0.25 ug Qfluor™ 710 Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human PBMCs were stained with 5 uL  (solid line) or 1.0 ug V450 Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human PBMCs were stained with 5 uL  (solid line) or 0.125 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human PBMCs were stained with 5 uL  .Human PBMCs were stained with 5 uL  (solid line) or 0.25 ug Mouse IgG1 FITC isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human PBMCs were stained with 5 uL  .Human PBMCs were stained with 5 uL  .Human PBMCs were stained with 5 uL  (solid line) or 0.25 ug Qfluor™ 710 Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human PBMCs were stained with 5 uL  (solid line) or 1.0 ug V450 Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Anti-CD45 Antibody [HI30]

$ 49.00$ 439.00
Please Select Product Options Below To View The Catalog Number.

SKU: QAB46
Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: PE ;Unconjugated ;APC ;APC-Cy7 ;FITC ;PerCP-Cy5.5 ;PE-Cy7 ;Qfluor 710 ;V450
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
QAB46-PE-25Tests Size: 25 tests, Tag: PE $ 49.00
QAB46-100ug Size: 100 ug, Tag: Unconjugated $ 79.00
QAB46-APC-100Tests Size: 100 tests, Tag: APC $ 169.00
QAB46-APC-25Tests Size: 25 tests, Tag: APC $ 79.00
QAB46-APC-500Tests Size: 500 tests, Tag: APC $ 439.00
QAB46-APC7-100Tests Size: 100 tests, Tag: APC-Cy7 $ 219.00
QAB46-APC7-25Tests Size: 25 tests, Tag: APC-Cy7 $ 119.00
QAB46-F-100Tests Size: 100 tests, Tag: FITC $ 109.00
QAB46-F-25Tests Size: 25 tests, Tag: FITC $ 59.00
QAB46-F-500Tests Size: 500 tests, Tag: FITC $ 359.00
QAB46-PCP55-100Tests Size: 100 tests, Tag: PerCP-Cy5.5 $ 269.00
QAB46-PCP55-25Tests Size: 25 tests, Tag: PerCP-Cy5.5 $ 109.00
QAB46-PE-100Tests Size: 100 tests, Tag: PE $ 109.00
QAB46-PE7-100Tests Size: 100 tests, Tag: PE-Cy7 $ 219.00
QAB46-PE7-25Tests Size: 25 tests, Tag: PE-Cy7 $ 109.00
QAB46-QF710-100Tests Size: 100 tests, Tag: Qfluorâ„¢ 710 $ 99.00
QAB46-QF710-25Tests Size: 25 tests, Tag: Qfluorâ„¢ 710 $ 49.00
QAB46-V450-100Tests Size: 100 tests, Tag: V450 $ 239.00
QAB46-V450-25Tests Size: 25 tests, Tag: V450 $ 109.00
QAB46-QF630-100ug Size: 100 tests, Tag: Qfluor™ 630 $ 159.00
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
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SKU: QAB46-PE-25Tests
CD45 General Information
Alternate Names
CD45, PTPRC, Lyt-4, T2, loc, CD45R, Ly-5, B22, Cd45, L-CA, LCA
Curated Database and Bioinformatic Data
Gene SymbolPTPRC
Entrez Gene ID5788
Ensemble Gene IDENSG00000081237, ENSG00000262418
RefSeq Protein Accession(s)XP_006711537, XP_006711536, XP_006711535, NP_563578, NP_001254727, NP_002829
RefSeq mRNA Accession(s)XM_006711473, XM_006711474, NM_080921, NM_080922, NM_001267798, NM_002838, XM_006711472, NR_052021
RefSeq Genomic Accession(s)NG_007730, NC_000001, NW_003315907, NC_018912
UniProt ID(s)X6R433, A0A0A0MT22, P08575, M9MML4
UniGene ID(s)X6R433, A0A0A0MT22, P08575, M9MML4
HGNC ID(s)9666
Cosmic ID(s)PTPRC
KEGG Gene ID(s)hsa:5788
PharmGKB ID(s)PA34011
General Description of CD45.
The HI30 antibody reacts with human CD45, one of the most abundant hematopoietic markers and one that is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.The HI30 antibody is widely used as a marker for human CD45 expression on T cells, B cells, monocytes, macrophages, and NK cells.

Human Anti-CD45 Antibody Product Attributes

Species: Human
Cross Reactivity: chimpanzee
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD45 Antibody Clone: HI30
Clone HI30 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD45 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD45 / PTPRC antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of CD45 / PTPRC could be seen in. We were unable to detect CD45 / PTPRC in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD45 expression as measured by anti-CD45 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - +++ - - - - - - - - - -
PTPRC Variability + + + + + + + + + + + + + + + + + + + +

Selected References

Strowig T, Rongvaux A, Rathinam C, Takizawa H, Borsotti C, Philbrick W, Eynon EE, Manz MG, and Flavell RA. 2011. Proc. Natl. Acad. Sci. 108: 13218-13223. (Flow Cytometry)Kim M-H, Suh H-S, Si Q, Terman BE, and Lee SC. 2006. J. Virol. 80: 62-72. (in vitro blocking, Western Blot)Zhang M and Varki A. 2004. Glycobiology. 14: 939-949. (Immunoprecipitation)Gelbmann CM, Leeb SN, Vogl D, Maendel M, Herfarth H, Scholmerich J, Falk W, and Rogler G. 2003. Gut. 52:1448-1456. (Immunocytochemistry)Yamada T, Zhu D, Saxon A, and Zhang K. 2002. J. Biol. Chem. 277(32): 28830-28835. (in vitro blocking)Esser MT, Graham DR, Coren LV, Trubey CM, Bess JW, Arthur LO, Ott DE, and Lifson JD. 2001. J. Virol. 75(13)6173-6182. (Western Blot)Goto E, Kohrogi H, Hirata N, Tsumori K, Hirosako S, Hamamoto J, Fujii K, Kawano O, and Ando M. 2000. Am. J. Respir. Cell Mol. Biol. 22: 405. (Immunohistochemistry - frozen tissue)Esser MT, Graham DR, Coren LV, Trubey CM, Bess JW, Arthur LO, Ott DE, and Lifson JD. 2001. J. Virol. 75(13)6173-6182. (Western Blot)

Limitations and Warranty

enQuire Bio's Human Anti-CD45 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD45 Antibody [HI30]

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