Human Anti-CD56 Antibody Product Attributes
Species: Human
Cross Reactivity: chimpanzee, cynomolgus monkey, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD56 Antibody Clone: MY31
Clone MY31 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD56 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Staining with anti-CD56 antibody reveals CD56 expression is expected to be primarily localized to the cytosol and plasma membrane.Observed Antibody Staining Data By Tissue Type:
Variations in CD56 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in neuropil in cerebral cortex and peripheral nerve/ganglion in colon. More moderate antibody staining intensity was present in neuropil in cerebral cortex and peripheral nerve/ganglion in colon. Low, but measureable presence of CD56 could be seen inglial cells in the hippocampus, exocrine glandular cells in the pancreas and smooth muscle cells in the smooth muscle. We were unable to detect CD56 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of CD56 expression as measured by anti-CD56 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | - | - | - | - | - | ++ | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| NCAM1 Variability | + | ++ | + | + | + | ++ | + | + | + | + | + | ++ | + | + | + | + | + | + | ++ | + |
Selected References
Lanier LL, Testi R, Bindl J and Phillips JH. 1989. J Exp Med. 169: 2233-2238.Schlossman SF, Boumsell L, Gilks W et al., eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.Carter DL, Shieh TM, Blosser RL, Chadwick KR, Margolick JB, Hidreth JE, Clements JE and Zink MC. 1999. Cytometry. 37(1): 41-50.Chan WK, Suwannasaen D, Throm RE, Li Y, Eldridge PW, Houston J, Gray JT, Pui C-H and Leung W. 2015. Leukemia. 29: 387-395. (Flow cytometry)Woltman AM, Op den Brouw ML, Biesta PJ, Shi CC and Janssen HLA. 2011. PLoS ONE 6(1): e15324. doi: 10.1371/journal.pone.0015324. (Flow cytometry)Bleul CC, Wu L, Hoxie JA, Springer TA and Mackay CR. 1997. Proc Natl Acad Sci USA. 94(5): 1925-1930. (Flow cytometry)Brown K and Barratt-Boyes SM. 2009. J Med Primatol. 38(4): 272-278. (Flow cytometry - Rhesus)
Limitations and Warranty
enQuire Bio's Human Anti-CD56 / NCAM Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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