Human PBMCs were stained with 5 uL (solid line) or 0.06 ug APC Mouse IgG2a isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human PBMCs were stained with 5 uL (solid line) or 0.06 ug Mouse IgG2a PE isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human PBMCs were stained with 5 uL (solid line) or 0.125 ug V450 Mouse IgG2a isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Anti-CD8a Antibody [OKT8]

Name: Anti-CD8a Antibody [OKT8]
Brand: enQuire Bio
Price: 59 USD
Availability: InStock

$ 59.00 – $ 739.00

Please Select Product Options Below To View The Catalog Number.

SKU: QAB17
Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;APC ;PerCP-Cy5.5 ;PE ;PE-Cy7 ;V450
Isotype: Mouse IgG2a
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
QAB17-F-25Tests	Size: 25 tests, Tag: FITC	$ 59.00
QAB17-APC-100Tests	Size: 100 tests, Tag: APC	$ 199.00
QAB17-APC-25Tests	Size: 25 tests, Tag: APC	$ 89.00
QAB17-APC-500Tests	Size: 500 tests, Tag: APC	$ 739.00
QAB17-F-100Tests	Size: 100 tests, Tag: FITC	$ 139.00
QAB17-F-500Tests	Size: 500 tests, Tag: FITC	$ 589.00
QAB17-PCP55-100Tests	Size: 100 tests, Tag: PerCP-Cy5.5	$ 219.00
QAB17-PCP55-25Tests	Size: 25 tests, Tag: PerCP-Cy5.5	$ 99.00
QAB17-PE-100Tests	Size: 100 tests, Tag: PE	$ 169.00
QAB17-PE-25Tests	Size: 25 tests, Tag: PE	$ 79.00
QAB17-PE-500Tests	Size: 500 tests, Tag: PE	$ 739.00
QAB17-PE7-100Tests	Size: 100 tests, Tag: PE-Cy7	$ 199.00
QAB17-PE7-25Tests	Size: 25 tests, Tag: PE-Cy7	$ 79.00
QAB17-V450-100Tests	Size: 100 tests, Tag: V450	$ 229.00
QAB17-V450-25Tests	Size: 25 tests, Tag: V450	$ 99.00

Shipping Information

In Stock.

Flat Rate Shipping Anywhere in the US: $45

Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: QAB17-F-25Tests

CD8 General Information
Alternate Names
CD8A, MAL, CD8, p32, Leu2, MAL
Curated Database and Bioinformatic Data
Gene Symbol	CD8A
Entrez Gene ID	925
Ensemble Gene ID	ENSG00000153563
RefSeq Protein Accession(s)	NP_001759, NP_001139345, NP_741969
RefSeq mRNA Accession(s)	NM_001145873, NM_001768, NM_171827, NR_027353,
RefSeq Genomic Accession(s)	NC_000002, NG_011608, NC_018913
UniProt ID(s)	P01732, Q8TAW8, Q6ZVS2
UniGene ID(s)	P01732, Q8TAW8, Q6ZVS2
HGNC ID(s)	1706
Cosmic ID(s)	CD8A
KEGG Gene ID(s)	hsa:925
PharmGKB ID(s)	PA26244
General Description of CD8.
The OKT8 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites. The OKT8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. If used together with alternative antibodies Human Anti-CD8a clone RPA-T8 or Human Anti-CD8a clone Hit8a, the OKT8 antibody will not block binding of RPA-T8 or Hit8a.

Human Anti-CD8 Antibody Product Attributes

Species: Human

Tested Applications: Flow Cytometry.

Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x10⁵ to 1x10⁸ cells.

Clonality: Monoclonal Antibody

Anti-CD8 Antibody Clone: OKT8

Clone OKT8 Host and Isotype: Mouse IgG2a

Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2

Antibody Concentration: 5 uL /test

Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD8 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD8 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the red pulp in spleen, cells in the white pulp in spleen, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the red pulp in spleen, cells in the white pulp in spleen, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD8 could be seen inmacrophages in lung. We were unable to detect CD8 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Jahnke M, Trowsdale J, and Kelly AP. 2012. J. Biol. Chem. 287: 28779-28789. (Flow Cytometry, Immunoprecipitation) Clement M, Ladell K, Ekeruche-Makinde J, Miles JJ, Edwards ESJ, Dolton G, Williams T, Schauenburg AJA, Cole DK, Lauder SN, Gallimore AM, Godkin AJ, Burrows SR, Price DA, Sewell AK, and Wooldridge L. 2011. J. Immunol. 187: 654-663. (in vitro activation) Bagnara D, Kaufman MS, Calissano C, Marsilio S, Patten PEM, Simone R, Chum P, Yan X-Y, Allen SL, Kolitz JE, Baskar S, Radar C, Mellstedt H, Rabbani H, Lee A, Gregersen PK, Rai KR, and Chiorazzi N. 2011. Blood. 117: 5463-5472. (in vivo activation) Teles RMB, Krutzik SR, Ochoa MT, Oliveira RB, Sarno EN, and Modlin RL. 2010. 78: 4634-4643. (Immunohistochemistry - OCT embedded frozen tissue) Lai AY, Fatemi M, Dhasarathy A, Malone C, Sobol SE, Geigerman C, Jaye DL, Mav D, Shah R, Li L, and Wade PA. 2010. J. Exp. Med. 207: 1939-1950. (in vitro T cell depletion) Varghese JC and Kane KP. 2008. J. Immunol. 181: 6002-6009. (in vitro blocking) Thakral D, Dobbins J, Devine L, and Kavathas PB. 2008. J. Immunol. 180:7431-7442. (Immunoprecipitation)

Limitations and Warranty

enQuire Bio's Human Anti-CD8a Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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