C57Bl/6 splenocytes were unstimulated  (solid line) or 0.06 ug APC Rat IgG2a isotype control (dashed line). Flow Cytometry Data from 10,000 events.
C57Bl/6 splenocytes were unstimulated  (solid line) or 0.06 ug APC Rat IgG2a isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were unstimulated  (solid line) or 0.125 ug PE Rat IgG2a isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stimulated for 3 days with LPS and then stained with APC Mouse Anti-CD19  and 0.25 ug PE-Cy7 Mouse Anti-CD86 .C57Bl/6 splenocytes were unstimulated  (solid line) or 0.06 ug APC Rat IgG2a isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were unstimulated  (solid line) or 0.125 ug PE Rat IgG2a isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stimulated for 3 days with LPS and then stained with APC Mouse Anti-CD19  and 0.25 ug PE-Cy7 Mouse Anti-CD86 .

Anti-CD86 Antibody [GL-1]

$ 58.00$ 368.00
Please Select Product Options Below To View The Catalog Number.

SKU: QAB51
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;Unconjugated ;APC ;PE ;PE-Cy7
Isotype: Rat IgG2a kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
QAB51-F-25ug Size: 25 ug, Tag: FITC $ 49.00
QAB51-100ug Size: 100 ug, Tag: Unconjugated $ 99.00
QAB51-APC-100ug Size: 100 ug, Tag: APC $ 219.00
QAB51-APC-25ug Size: 25 ug, Tag: APC $ 79.00
QAB51-F-100ug Size: 100 ug, Tag: FITC $ 129.00
QAB51-F-500ug Size: 500 ug, Tag: FITC $ 209.00
QAB51-PE-100ug Size: 100 ug, Tag: PE $ 169.00
QAB51-PE-25ug Size: 25 ug, Tag: PE $ 59.00
QAB51-PE7-100ug Size: 100 ug, Tag: PE-Cy7 $ 329.00
QAB51-PE7-25ug Size: 25 ug, Tag: PE-Cy7 $ 99.00
QAB51-QF630-100ug Size: 100 tests, Tag: Qfluor™ 630 $ 179.00
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

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Purity:

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SKU: QAB51-F-25ug
CD86 General Information
Alternate Names
B7-2, B7.2
Curated Database and Bioinformatic Data
Gene SymbolCd86
Entrez Gene ID12524
Ensemble Gene IDENSMUSG00000022901
RefSeq Protein Accession(s)XP_011244114, XP_006521804, NP_062261
RefSeq mRNA Accession(s)XM_011245812, XM_006521741, XR_384517, NM_019388
RefSeq Genomic Accession(s)NC_000082
UniProt ID(s)Q549Q9, Q61238, P42082
UniGene ID(s)Q549Q9, Q61238, P42082
Cosmic ID(s)Cd86
KEGG Gene ID(s)mmu:12524
General Description of CD86.
The GL-1 antibody reacts with mouse CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 can also bind a second B7 ligand known as CD80 (B7-1). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. The GL-1 antibody may be used as a marker for CD86 expression on B cells, macrophages, and dendritic cells.

Mouse Anti-CD86 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD86 Antibody Clone: GL-1 (GL1)
Clone GL-1 (GL1) Host and Isotype: Rat IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD86 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD86 expression as measured by anti-CD86 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ ++ + ++ ++ + + ++ ++ ++ ++ + ++ + - + ++ ++ ++ ++
CD86 Variability ++ ++ ++ + ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + + ++

Selected References

Liu Z, Geboes K, Hellings P, Maerten P, Heremans H, Vandenberghe P, Boon L, van Kooten P, Rutgeerts P, and Ceuppens JL. 2011. J. Immunol. 167: 1830-1838. (in vivo blocking, Immunohistochemistry - OCT embedded frozen tissue)Kastenmuller W, Gasteiger G, Subramanian N, Sparwasser T, Busch DH, Belkaid Y, Drexler I, and Germain RN, 2011. J. Immunol. 187: 3186-3197. (in vivo blocking)Zheng SG, Wang JH, Stohl, W, Kim KS, Gray JD, and Horwitz DA. 2006. J. Immunol. 176:3321-3329. (in vitro blocking)Leithauser F, Meinhardt-Krajina T, Fink K, Wotschke B, Moller P and Reimann J. 2006. Am. J. Pathol. 168(6): 1898-1909. (Immunohistochemistry - frozen tissue)Odobasic D, Kitching AR, Semple TJ, Timoshanko JR, Tipping PG, and Holdsworth SR. 2005. J. Am. Soc. Nephrol. 16: 2012-2022. (in vivo activation, Immunofluorescence microscopy - frozen tissue, Immunohistochemistry - frozen tissue)Lenschow DJ, Ho SC, Sattar H, Rhee L, Gray G, Nabavi N,Herold KC, and Bluestone JA. 1995. J. Exp. Med. 181:1145-155. (in vitro blocking)Blazar BR, Taylor PA, Panoskaltsis-Mortari A, Gray GS, and Vallera DA. 1995. Blood. 85: 2607-2618. (Immunohistochemistry - OCT embedded frozen tissue)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD86 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD86 Antibody [GL-1]

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