Human, Mouse, and Rat Anti-CD86 Antibody Product Attributes
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (2-4ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD86 Antibody Clone: SPM600
Clone SPM600 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse, and Rat CD86 Positive Control Sample: 293T or Jukat cells. Monocytes, dendritic cells, activated T, B, natural killer cells in lymph node or tonsil.
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human full-length CD86 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
CD86 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of CD86 expression as measured by anti-CD86 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | ++ | ++ | + | ++ | ++ | + | + | ++ | ++ | ++ | ++ | + | ++ | + | - | + | ++ | ++ | ++ | ++ |
CD86 Variability | ++ | ++ | ++ | + | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | + | + | ++ |
Limitations and Warranty
enQuire Bio's CD86 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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