Human and Mouse Anti-Cytokeratin 6 Antibody Product Attributes
Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.1-0.2ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cytokeratin 6 Antibody Clone: SPM269
Clone SPM269 Host and Isotype: Mouse IgG2a kappa
Anti-Human and Mouse Cytokeratin 6 Positive Control Sample: Suprabasal cells of the outer hair follicle root sheath in skin. Basal cell carcinoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from C-terminus of human keratin 6
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Cytokeratin 6 Previously Observed Antibody Staining Patterns
| Cytokeratin 6 General Information | |
|---|---|
| Alternate Names | |
| CK6A, CK6B, CK6C, CK6D, CK6E, Keratin Type II Cytoskeletal 6A, Keratin Type II Cytoskeletal 6B, Keratin Type II Cytoskeletal 6C, Keratin Type II Cytoskeletal 6D, Keratin Type II Cytoskeletal 6E, KRT6, KRT6A, KRT6B, KRT6C, KRT6D, KRT6E, anti-CK6A antibody, anti-CK6B antibody, anti-CK6C antibody, anti-CK6D antibody, anti-CK6E antibody, anti-Keratin Type II Cytoskeletal 6A antibody, anti-Keratin Type II Cytoskeletal 6B antibody, anti-Keratin Type II Cytoskeletal 6C antibody, anti-Keratin Type II Cytoskeletal 6D antibody, anti-Keratin Type II Cytoskeletal 6E antibody, anti-KRT6 antibody, anti-KRT6A antibody, anti-KRT6B antibody, anti-KRT6C antibody, anti-KRT6D antibody, anti-KRT6E antibody | |
| Molecular Weight | |
| 56kDa | |
| Chromosomal Location | |
| 12q13.13; 12q13.13; 12q13.13 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | KRT6A; KRT6B; KRT6C |
| Entrez Gene ID | 3853, 3854, 286887 |
| Ensemble Gene ID | ENSG00000205420, ENSG00000185479, ENSG00000170465 |
| RefSeq Protein Accession(s) | NP_005545, NP_005546, NP_775109 |
| RefSeq mRNA Accession(s) | NM_005554, NM_005555, NM_173086, |
| RefSeq Genomic Accession(s) | NG_008298, NC_000012, NC_018923, NG_008299, NC_018923, NC_000012, NC_000012, NC_018923, NG_012416 |
| UniProt ID(s) | P02538, A0A0S2Z428, P04259, P48668 |
| UniGene ID(s) | P02538, A0A0S2Z428, P04259, P48668 |
| HGNC ID(s) | 6443, 6444, 20406 |
| Cosmic ID(s) | KRT6A, KRT6B, KRT6C |
| KEGG Gene ID(s) | hsa:3853, hsa:3854, hsa:286887 |
| PharmGKB ID(s) | PA30231, PA30232, PA134891227 |
| General Description of Cytokeratin 6. | |
| This MAb recognizes a protein of 56kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns,, Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13,, mutations in this gene are linked to several inheritable hair, skin pathologies. Keratins 6, 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal, hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6, CK16. CK6 is strongly expressed in about 75% of head, neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation. | |
Limitations and Warranty
enQuire Bio’s Cytokeratin 6 Anti-Human, Mouse Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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