Formalin-fixed, paraffin embedded human tonsil sections stained with 100 ul anti-IgG (clone SPM556) at 1:300. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.
Formalin-fixed, paraffin embedded human tonsil sections stained with 100 ul anti-IgG (clone SPM556) at 1:300. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.

Anti-IgG Antibody Clone SPM556

$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 3500-MSM2X
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
3500-MSM2X-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 3500-MSM2X-P1
IgG General Information
Alternate Names
IGHG, Immunoglobulin Heavy Chain (Gamma Polypeptide)
Molecular Weight
75kDa
Chromosomal Location
14q32.33
Curated Database and Bioinformatic Data
Gene SymbolIGHG
Entrez Gene ID3500, 3501, 3502, 3503
RefSeq Genomic Accession(s)NG_187600, NC_000014, NG_001019, NG_001019, NG_187600, NC_018925, NC_000014NC_000014, NG_001019, NG_187600, NC_000014, NG_001019, NC_018925, NG_187600
UniGene ID(s)
HGNC ID(s)5525, 5526, 5527, 5528
Cosmic ID(s)IGHG1, IGHG2, IGHG3, IGHG4
General Description of IgG.
Recognizes a protein of 75kDa, identified as γ heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), μ (IgM), ε (IgE), or δ (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas,, certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal, therefore malignant.

Human Anti-IgG Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-IgG Antibody Clone: SPM556
Clone SPM556 Host and Isotype: Mouse IgG2a kappa
Anti-Human IgG Positive Control Sample: 293T, Raji or hPBL cells. Tonsil or Spleen.
Cellular Localization of Antibody Cytoplasm, Cell Surface, Secreted
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Purified human Ig Gamma Chain
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

IgG Previously Observed Antibody Staining Patterns

Limitations and Warranty

enQuire Bio's IgG Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-IgG Antibody Clone SPM556

https://enquirebio.com/wp-content/uploads/2019/01/IgG IHC human Tonsil (SPM556)-300x225.jpg