Formalin-fixed, paraffin embedded human tonsil sections stained with 100 ul anti-Kappa Light Chain (clone HP6053) at 1:200. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.
Formalin-fixed, paraffin embedded human tonsil sections stained with 100 ul anti-Kappa Light Chain (clone HP6053) at 1:200. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.

Anti-Kappa Light Chain Antibody Clone HP6053

$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 3514-MSM4
Species: Human
Tested Applications: Flow Cytometry, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
3514-MSM4-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 3514-MSM4-P1
Kappa Light Chain General Information
Alternate Names
Immunoglobulin kappa constant, IGKC
Molecular Weight
~22.5kDa
Chromosomal Location
2p11.2
Curated Database and Bioinformatic Data
Gene SymbolIGKC
Entrez Gene ID3514
RefSeq Genomic Accession(s)NC_000002, NG_000834, NC_018913
UniGene ID(s)
HGNC ID(s)5716
Cosmic ID(s)IGKC
General Description of Kappa Light Chain.
This MAb is specific to kappa light chain of immunoglobulin, shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas,, certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Human Anti-Kappa Light Chain Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-2.0ug of antibody/million cells in 0.1ml), Western Blot (0.5-1.0ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Kappa Light Chain Antibody Clone: HP6053
Clone HP6053 Host and Isotype: Mouse IgG1 kappa
Anti-Human Kappa Light Chain Positive Control Sample: 293T, Raji or hPBL cells. Tonsil or Spleen
Cellular Localization of Antibody Cell Surface, cytoplasmic, secreted
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Purified human Ig kappa chain
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Kappa Light Chain Previously Observed Antibody Staining Patterns

Limitations and Warranty

enQuire Bio's Kappa Light Chain Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Kappa Light Chain Antibody Clone HP6053

https://enquirebio.com/wp-content/uploads/2019/01/Kappa Light Chain IHC human Tonsil (HP6053)-300x225.jpg