Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with RBP1 (G4E4)

Anti-Retinol Binding Protein-1 Antibody Clone G4E4

Name: Anti-Retinol Binding Protein-1 Antibody Clone G4E4
Brand: enQuire Bio
Price: 429 USD
Availability: InStock

$ 429.00

Please Select Product Options Below To View The Catalog Number.

SKU: 5947-MSM1
Species: Human, Chimpanzee, Monkey, Goat, Rabbit, Rat, and Mouse
Tested Applications: Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
5947-MSM1-P1	Size: 100 ug, Tag: Unconjugated

Shipping Information

In Stock.

Flat Rate Shipping Anywhere in the US: $45

Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: 5947-MSM1-P1

Retinol Binding Protein-1 General Information
Alternate Names
Retinol binding protein 1, cellular RBP1, RBP1
Molecular Weight
21-25kDa
Chromosomal Location
10q23.33
Curated Database and Bioinformatic Data
Gene Symbol	RBP1
Entrez Gene ID	5947, 5948, 5950
Ensemble Gene ID	ENSG00000114115, ENSG00000114113, ENSG00000138207
RefSeq Protein Accession(s)	NP_001124464, NP_002890, NP_001124465, NP_004155, NP_001310446, NP_001310447, NP_006735
RefSeq mRNA Accession(s)	NM_002899, NM_001130993, NM_001130992, NM_004164, NM_001323517, NM_001323518, NM_006744,
RefSeq Genomic Accession(s)	NC_018914, NG_047073, NC_000003, NC_000003, NC_018914 NC_000010, NC_018921, NG_009104
UniProt ID(s)	A0A0A0MQT0, P09455, P50120, Q5VY30, P02753
UniGene ID(s)	A0A0A0MQT0, P09455, P50120, Q5VY30, P02753
HGNC ID(s)	9919, 9920, 9922
Cosmic ID(s)	RBP1, RBP2, RBP4
KEGG Gene ID(s)	hsa:5947, hsa:5948, hsa:5950
PharmGKB ID(s)	PA34286, PA34287, PA34289
General Description of Retinol Binding Protein-1.
This MAb recognizes an epitope within the 74-182 C-terminal sequence (11kD peptide fragment) of human serum Cellular Retinol Binding Protein 1 (CRBP 1), a single-chain glycoprotein belonging to the superfamily of hydrophobic molecule transporter proteins, which is responsible for transport of retinol (vitamin A1) from the liver to peripheral target tissues, like the eye, where it mediates the cellular uptake. CRBP 1 is synthesized by hepatic parenchymal cells where it becomes bound to its ligand retinol, is then released into the circulation, where it binds further to the protein transthyretin, to form a transporting complex, which is big enough not to be lost by filtration through the kidney glomeruli. It is detected in nearly all tissues with higher expression in adult ovary, pancreas, pituitary gland, adrenal gland,, fetal liver.

Human, Chimpanzee, Monkey, Goat, Rabbit, Rat, and Mouse Anti-Retinol Binding Protein-1 Antibody Product Attributes

Species: Human, Chimpanzee, Monkey, Goat, Rabbit, Rat, and Mouse

Tested Applications: Immunofluorescence, Immunohistochemistry (IHC).

Application Notes: Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)

Clonality: Monoclonal

Anti-Retinol Binding Protein-1 Antibody Clone: G4E4

Clone G4E4 Host and Isotype: Mouse IgG1 kappa

Anti-Human, Chimpanzee, Monkey, Goat, Rabbit, Rat, and Mouse Retinol Binding Protein-1 Positive Control Sample: Hepatic or ovarian carcinoma

Cellular Localization of Antibody Cytoplasmic

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.

Antibody Concentration: 200ug/ml

Antibody Purification Method:Protein A/G Purified

Immunogen: Retinol binding protein-1 purified from human plasma

Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.

Retinol Binding Protein-1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-RBP1 antibody staining is expected to be primarily localized to the cytosol and nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in Retinol Binding Protein-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in Leydig cells in the testis. More moderate antibody staining intensity was present in Leydig cells in the testis. Low, but measureable presence of Retinol Binding Protein-1 could be seen inglandular cells in the breast, glial cells in the caudate nucleus, cells in the molecular layer in cerebellum, glandular cells in the cervix, uterine, cells in the endometrial stroma in endometrium, glandular cells in the gallbladder, glial cells in the hippocampus, cells in the tubules in kidney and cells in the seminiferous ducts in testis. We were unable to detect Retinol Binding Protein-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Retinol Binding Protein-1 expression as measured by anti-Retinol Binding Protein-1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	-	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-
RBP1 Variability	+	++	+	++	++	++	++	+	++	+	+	++	++	+	+	++	+	++	+	++

Limitations and Warranty

enQuire Bio's Retinol Binding Protein-1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.