Western blot analysis of human brain lysate using S1B Mouse Monoclonal Antibody (4C4.9).
Western blot analysis of human brain lysate using S1B Mouse Monoclonal Antibody (4C4.9).Confocal Immunofluorescent analysis of A258 cells using AF488-labeled S1B Monoclonal Antibody (4C4.9) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).Confocal Immunofluorescent analysis of A258 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)

Anti-S100B Antibody Clone 4C4.9

$ 118.00$ 418.00
Please Select Product Options Below To View The Catalog Number.

SKU: 6285-MSM1
Species: Human
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
6285-MSM1-P0 Size: 20 ug, Tag: Unconjugated $ 118.00
6285-MSM1-P1 Size: 100 ug, Tag: Unconjugated $ 298.00
6285-MSM1-P2 Size: 200 ug, Tag: Unconjugated $ 418.00
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SKU: 6285-MSM1-P0
S100B General Information
Alternate Names
S100 calcium-binding protein B, S100B
Molecular Weight
10-12kDa
Chromosomal Location
21q22.3
Curated Database and Bioinformatic Data
Gene SymbolS100B
Entrez Gene ID6285
Ensemble Gene IDENSG00000160307
RefSeq Protein Accession(s)NP_006263, XP_016883913
RefSeq mRNA Accession(s)XM_017028424, NM_006272
RefSeq Genomic Accession(s)NC_018932, NC_000021
UniProt ID(s)A0A0S2Z4C5, P04271
UniGene ID(s)A0A0S2Z4C5, P04271
HGNC ID(s)10500
Cosmic ID(s)S100B
KEGG Gene ID(s)hsa:6285
PharmGKB ID(s)PA34912
General Description of S100B.
S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

Human Anti-S100B Antibody Product Attributes

Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-S100B Antibody Clone: 4C4.9
Clone 4C4.9 Host and Isotype: Mouse IgG2a kappa
Anti-Human S100B Positive Control Sample: Brain, Melanoma
Cellular Localization of Antibody Cytoplasmic and Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Purified bovine brain S100 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

S100B Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-S100B antibody staining is expected to be primarily localized to the nucleus and vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in S100B antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in myoepithelial cells in the breast, glial cells in the caudate nucleus, neuronal cells in the caudate nucleus, cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, Purkinje cells in the cerebellum, glial cells in the cerebral cortex, neuronal cells in the cerebral cortex, neuropil in cerebral cortex, peripheral nerve/ganglion in colon, glial cells in the hippocampus, neuronal cells in the hippocampus, Langerhans in skin and peripheral nerve in mesenchymal tissue. More moderate antibody staining intensity was present in myoepithelial cells in the breast, glial cells in the caudate nucleus, neuronal cells in the caudate nucleus, cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, Purkinje cells in the cerebellum, glial cells in the cerebral cortex, neuronal cells in the cerebral cortex, neuropil in cerebral cortex, peripheral nerve/ganglion in colon, glial cells in the hippocampus, neuronal cells in the hippocampus, Langerhans in skin and peripheral nerve in mesenchymal tissue. Low, but measureable presence of S100B could be seen in. We were unable to detect S100B in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of S100B expression as measured by anti-S100B antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ + - + + ++ - - - - ++ + ++ + - - + - + +
S100B Variability ++ ++ ++ ++ ++ ++ ++ ++ + + +++ ++ + ++ + + ++ ++ ++ ++

Limitations and Warranty

enQuire Bio's S100B Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-S100B Antibody Clone 4C4.9

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