Formalin-fixed, paraffin-embedded human Fetal Liver stained with Alexa Fluor® P Monoclonal Antibody (SPM334).

Anti-AFP Antibody Clone SPM334

Name: Anti-AFP Antibody Clone SPM334
Brand: enQuire Bio
Price: 429 USD
Availability: InStock

$ 488.00

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SKU: 174-MSM1X
Species: Human, Monkey, Canine, Pig, Cow (-), Cat (-), Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
174-MSM1X-P1	Size: 100 ug, Tag: Unconjugated

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Datasheets and Documentation

Product Datasheet

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SKU: 174-MSM1X-P1

AFP General Information
Alternate Names
Alpha-fetoprotein, AFP, ?-fetoprotein
Molecular Weight
70kDa
Chromosomal Location
4q13.3
Curated Database and Bioinformatic Data
Gene Symbol	AFP
Entrez Gene ID	174
Ensemble Gene ID	ENSG00000081051
RefSeq Protein Accession(s)	NP_001125, NP_001341646
RefSeq mRNA Accession(s)	NM_001134, NM_001354717
RefSeq Genomic Accession(s)	NC_000004, NG_023028, NC_018915
UniProt ID(s)	P02771
UniGene ID(s)	P02771
HGNC ID(s)	317
Cosmic ID(s)	AFP
KEGG Gene ID(s)	hsa:174
PharmGKB ID(s)	PA24614
General Description of AFP.
It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP, shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract,, yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC), germ cell neoplasms, especially yolk sac tumors.

Human, Monkey, Canine, Pig, Cow (-), Cat (-), Mouse (-), and Rat (-) Anti-AFP Antibody Product Attributes

Species: Human, Monkey, Canine, Pig, Cow (-), Cat (-), Mouse (-), and Rat (-)

Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).

Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)

Clonality: Monoclonal

Anti-AFP Antibody Clone: SPM334

Clone SPM334 Host and Isotype: Mouse IgG2a kappa

Anti-Human, Monkey, Canine, Pig, Cow (-), Cat (-), Mouse (-), and Rat (-) AFP Positive Control Sample: Hep-G2 cells. Fetal liver or hepatocellular carcinoma

Cellular Localization of Antibody Cytoplasmic

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.

Antibody Concentration: 200ug/ml

Antibody Purification Method:Protein A/G Purified

Immunogen: Alpha fetoprotein (AFP) purified from serum of a hepatoma patient

Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.

AFP Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-AFP antibody staining is expected to be primarily localized to the cytosol.

Observed Antibody Staining Data By Tissue Type:

Variations in AFP antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of AFP could be seen inglandular cells in the prostate, hepatocytes in liver and trophoblastic cells in the placenta. We were unable to detect AFP in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of AFP expression as measured by anti-AFP antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	+	+	+	+	+	+	+	++	+	+	+	++	+	+	+	+	+	++	+	+
AFP Variability	+	++	+	+	++	++	++	++	++	+	++	++	++	++	++	+	+	++	+	++

Limitations and Warranty

enQuire Bio's AFP Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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