Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:25-1:50 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-CA 125 Antibody Clone: OV185:1
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Ovarian carcinoma
Cellular Localization of Antibody OV185:1 Staining: Extracellular membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Partially purified human mucin fraction from a pool of cancer tissues of patients with epithelial ovarian cancer.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
CA 125 Information for Pathologists
Summary:
Cancer antigen 125 (CA-125) is a protein encoded by MUC16 gene, used as a tumor marker, because it is 79% sensitive for ovarian cancer. Not specific, because elevated in other tumors and in inflammatory conditions. Terminology Also known as MUC16. PathophysiologyNotable Clinical Features:
Elevated in carcinomas of breast, cervix (Asian Pac J Cancer Prev 2010;11:1745), endometrium, fallopian tubes, GI, lung, ovary; also endometriosis, pregnancy, inflammatory disorders. Interpretation Strong membranous staining, fallopian tube is good control. Uses by pathologists (1) For ovarian carcinomas, use serum levels to follow response to treatment and predict prognosis (Ann Oncol 2008;19:327).Common Uses By Pathologists:
(1) For ovarian carcinomas, use serum levels to follow response to treatment and predict prognosis (Ann Oncol 2008;19:327). (2) In UK, recommended to use serum levels to screen women with symptoms of ovarian cancer (National Health Service Press Release). (3) Serial measurements may be more specific for ovarian cancer. (4) Useful in immunohistochemistry to confirm ovarian origin of tumor (Am J Surg Pathol 2005;29:1482). (5) Serum levels may have role in assessing heart failure (Clin Cardiol 2011;34:244, Eur Heart J 2010;31:1752).Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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