Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
|Recommended Dilution (Conc)
||EDTA Buffer pH 8.0
||30 min at Room Temperature
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Anti-CD15/Granulocyte Associated Antibody Clone: MMA; same as LeuM1
Host and Isotype: Mouse IgM, kappa
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody MMA; same as LeuM1 Staining: Cell membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Lot specific. Plese contact tech support
Immunogen: BALB/C mice were injected with U937 histiocytic cell line.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
CD15/Granulocyte Associated Information for Pathologists
CD15 is a A carbohydrate (most traditional diagnostic antigens are proteins) that is widely used for diagnosis of Hodgkin lymphoma. CD15 is also known as LeuM1 or Lewis X. Normal physiological roles for CD15 include mediating phagocytosis and chemotaxis of immune cells.
Notable Clinical Features:
Acute promyelocytic leukemia CD15+ staining may indicate poor prognosis. CD15 may be useful to differentiate recent injuries from post mortem damage. (Int J Legal Med 2013;127:957). In addition to its role in Hodgkin's diagnosis, CD15 may differentiate pulmonary adenocarcinoma (CD15+) from mesothelioma (CD15-), although other markers are more specific. It also may help define neural stem cells.
Common Uses By Pathologists:
For Hodgkin lymphoma CD15 staining is used to confirm diagnosis and differentiate Hodgkin lymphoma from anaplastic large cell lymphomas. Staining is generally membranous or diffuse cytoplasmic and is observed in the golgi of Reed-Sternberg cells.
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.