Immunohistochemical staining of CD15/Granulocyte Associated  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

CD15/Granulocyte Associated [MMA; same as LeuM1] Antibody (cGMP).

$ 135.00$ 405.00
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Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgM, kappa

Product NumberDescriptionPrice
QIVD53-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD53-0.5ml Size: 0.5 ml, Format: Concentrate $ 245.00
QIVD53-1ml Size: 1 ml, Format: Concentrate $ 405.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
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SKU: QIVD53-0.1ml
CD15/Granulocyte Associated General Information
Alternate Names
Curated Database and Bioinformatic Data
UniProt ID(s)Carbohydrate Antigen
General Description of CD15/Granulocyte Associated .
This antibody reacts with a 220 kDa protein. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. CD15 is also expressed in Reed-Sternberg cells and some epithelial cells. CD15 is very useful in the identification of Hodgkin disease.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment EDTA Buffer pH 8.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-CD15/Granulocyte Associated Antibody Clone: MMA; same as LeuM1
Host and Isotype: Mouse IgM, kappa
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody MMA; same as LeuM1 Staining: Cell membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with U937 histiocytic cell line.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

CD15/Granulocyte Associated Information for Pathologists


CD15 is a A carbohydrate (most traditional diagnostic antigens are proteins) that is widely used for diagnosis of Hodgkin lymphoma. CD15 is also known as LeuM1 or Lewis X. Normal physiological roles for CD15 include mediating phagocytosis and chemotaxis of immune cells.

Notable Clinical Features:

Acute promyelocytic leukemia CD15+ staining may indicate poor prognosis. CD15 may be useful to differentiate recent injuries from post mortem damage. (Int J Legal Med 2013;127:957). In addition to its role in Hodgkin's diagnosis, CD15 may differentiate pulmonary adenocarcinoma (CD15+) from mesothelioma (CD15-), although other markers are more specific. It also may help define neural stem cells.

Common Uses By Pathologists:

For Hodgkin lymphoma CD15 staining is used to confirm diagnosis and differentiate Hodgkin lymphoma from anaplastic large cell lymphomas. Staining is generally membranous or diffuse cytoplasmic and is observed in the golgi of Reed-Sternberg cells.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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CD15/Granulocyte Associated [MMA; same as LeuM1] Antibody (cGMP).