Mouse Fc-Block (Anti-CD16/CD32) Antibody Product Attributes
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD16/CD32 Antibody Clone: 2.4G2
Clone 2.4G2 Host and Isotype: Rat IgG2b
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
Anti-CD16/CD32 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD16 / CD32 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD16 / CD32 could be seen in cells in the seminiferous ducts in testis, epidermal cells in the skin, exocrine glandular cells in the pancreas, germinal center cells in the lymph node, glandular cells in the epididymis, fallopian tube, gallbladder, salivary gland, stomach and thyroid gland, lymphoid tissue in appendix, macrophages in lung, myocytes in heart muscle, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the cervix and uterine, oral mucosa and vagina. We were unable to detect CD16 / CD32 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Stephen TL, Wilson BS, and Laufer TM. 2012. Proc. Natl. Acad. Sci. 109: 7415-7420. (Blocking- Immunofluorescence microscopy)Yamaji O, Nagaishi T, Totsuka T, Onizawa M, Suzuki M, Tsuge et al. 2012. J. Immunol. 188:2524-2536. (Blocking - in vitro)Shimazu T, Iida R, Zhang Q, Welner RS, Medina KL, Alberola-Ila J, and Kincade PW. 2012. Blood. 119:4889-4897. (Blocking - Flow cytometry)Stoeker L, Nordone S, Gunderson S, Zhang L, Kajikawa A, LaVoy A, Miller M, Klaenhammer TR, and Dean GA. 2011. Clin. Vaccine Immunol. 18: 1834-1844. (Blocking - Flow cytometry)Beretta F, St.-Pierre J, Piccirillo CA, and Stevenson MM. 2011. J. Immunol. 186: 4862-4871. (Blocking - Flow cytometry)Coudert JD, Scarpellino L, Gros F, Vivier E, and Held W. 2008 Blood. 111: 3571-3578. (Immunoprecipitation)
Limitations and Warranty
enQuire Bio's Mouse Anti-CD32 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.