Human PBMCs were stained with 5 uL  (solid line) or 0.25 ug APC Mouse IgG2a isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD45RO Antibody [UCHL1]

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Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;Unconjugated ;APC ;Biotin ;PerCP-Cy5.5 ;PE ;APC-Cy7
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB44-F-25Tests
CD45RO General Information
Curated Database and Bioinformatic Data
Gene SymbolPTPRC
Entrez Gene ID5788
Ensemble Gene IDENSG00000081237, ENSG00000262418
RefSeq Protein Accession(s)XP_006711537, XP_006711536, XP_006711535, NP_563578, NP_001254727, NP_002829
RefSeq mRNA Accession(s)XM_006711473, XM_006711474, NM_080921, NM_080922, NM_001267798, NM_002838, XM_006711472, NR_052021
RefSeq Genomic Accession(s)NG_007730, NC_000001, NW_003315907, NC_018912
UniProt ID(s)X6R433, A0A0A0MT22, P08575, M9MML4
UniGene ID(s)X6R433, A0A0A0MT22, P08575, M9MML4
HGNC ID(s)9666
Cosmic ID(s)PTPRC
KEGG Gene ID(s)hsa:5788
PharmGKB ID(s)PA34011
General Description of CD45RO.
The UCHL1 antibody reacts with the human CD45 isoform known as CD45RO, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA and CD45RB.The UCHL1 antibody is widely used as a marker for human CD45RO expression on thymocytes, activated memory T cells, monocytes, macrophages and granulocytes. The antibody is reported to be cross-reactive for Chimpanzee CD45RO.

Human Anti-CD45RO Antibody Product Attributes

Species: Human
Cross Reactivity: chimpanzee
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD45RO Antibody Clone: UCHL1
Clone UCHL1 Host and Isotype: Mouse IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD45RO Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD45RO antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of CD45RO could be seen in. We were unable to detect CD45RO in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD45RO expression as measured by anti-CD45RO antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - +++ - - - - - - - - - -
PTPRC Variability + + + + + + + + + + + + + + + + + + + +

Selected References

Imanguli MM, Swaim WD, League SC, Gress RE, Pavletic SZ, and Hakim FT. 2009. Blood. 113: 3620-3630. (Immunohistochemistry - paraffin embedded tissue)Di Carlo E, D’Antuono T, Pompa P, Giuliani R, Rosini S, Stuppia L, Musiani P, and Sorrentino C. 2009. Clin. Cancer Res. 15: 2979-2987. (Immunohistochemistry - paraffin embedded tissue)Kap YS, van Meurs M, van Driel N, Koopman G, Melief M-J, Brok HPM, Laman JD, and ‘t Hart BA. 2009. J.Histochemistry & Cytochemistry. 57: 1159-1167. (Immunohistochemistry - Chimpanzee frozen tissue)Bonzheim I, Geissinger E, Tinguely M, Roth S, Grieb T, Reimer P, Wilhelm M, Rosenwald A, Muller-Hermelink HK, and Rudiger T. 2008. Am. J. Clin. Pathol. 130: 613-619. (Immunofluorescence microscopy - frozen tissue)Kim M-H, Suh H-S, Si Q, Terman BE, and Lee SC. 2006. J. Virol. 80: 62-72. (Western Blot)Cappione AJ, Pugh-Bernard AE, Anolik JH, and Sanz I. 2004. J. Immunol. 172: 4298-4307. (Immunoprecipitation)Gougeon ML, Lecoeur H, Boudet F, Ledru E. Marzabal S, Boullier S, Roue R, Nagata S, and Heeney J. 1997. J. Immunol. 158: 2964-2976. (Flow cytometry - Chimpanzee)Kulas DT, Freund GG, and Mooney RA. 1996. J. Biol. Chem. 271: 755-760. (Immunoprecipitation, Western Blot)

Limitations and Warranty

enQuire Bio's Human Anti-CD45RO Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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