Human, Monkey, Rabbit, Cat, Pig (-), Canine (-), and Chicken (-) Anti-CD68 Antibody Product Attributes
Species: Human, Monkey, Rabbit, Cat, Pig (-), Canine (-), and Chicken (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Anti-CD68 Antibody Clone: SPM130
Clone SPM130 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Monkey, Rabbit, Cat, Pig (-), Canine (-), and Chicken (-) CD68 Positive Control Sample: Tonsil, Lymph Node or Spleen
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Subcellular fraction of human alveolar macrophages
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
CD68 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-CD68 antibody staining is expected to be primarily localized to the vesicles and golgi apparatus.
Observed Antibody Staining Data By Tissue Type:
Variations in CD68 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the red pulp in spleen and macrophages in lung. More moderate antibody staining intensity was present in cells in the red pulp in spleen and macrophages in lung. Low, but measureable presence of CD68 could be seen inglial cells in the cerebral cortex. We were unable to detect CD68 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's CD68 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.