Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-Cdc47/MCM7 Antibody Clone: 47DC141
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Breast carcinoma
Cellular Localization of Antibody 47DC141 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with recombinant human cdc47 protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Cdc47/MCM7 Information for Pathologists
| Cdc47/MCM7 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 81.3 kDa | |
| Chromosomal Location | |
| q22.1 [chr: 7] [chr_start: 100092728] [chr_end: 100101940] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MCM7 |
| Entrez Gene ID | 4176 |
| RefSeq Protein Accession(s) | NP_001265524; NP_877577; NP_005907 |
| RefSeq mRNA Accession(s) | NM_182776; NM_001278595; XM_017012217; NM_005916; XM_005250348 |
| RefSeq Genomic Accession(s) | NC_000007 |
| UniProt ID(s) | P33993 |
| PharmGKB ID(s) | PA30697 |
| KEGG Gene ID(s) | hsa:4176 |
| General Description of Cdc47/MCM7 . | |
| This antibody reacts with an 80 kDa protein known as hCdc47. hCdc47 is a member of the MCM family, which allows DNA to replicate once per cell cycle. Expression of Cdc47 protein has been found in the nuclei of proliferative components of normal lymph nodes, bone marrow, epidermis and mucosa. Malignant tumors from several organs contain more nuclear hCdc47-positive cells than their normal counterparts. | |
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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