Human and Mouse Anti-Cyclin B1 Antibody Product Attributes
Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cyclin B1 Antibody Clone: SPM619
Clone SPM619 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Mouse Cyclin B1 Positive Control Sample: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.
Cellular Localization of Antibody Cytoplasmic & Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human full-length CCNB1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Cyclin B1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-CCNB1 antibody staining is expected to be primarily localized to the cytosol. There is variability in either the signal strength or the localization of signal in cytosol from cell to cell.Observed Antibody Staining Data By Tissue Type:
Variations in Cyclin B1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the seminiferous ducts in testis, germinal center cells in the lymph node and tonsil and hematopoietic cells in the bone marrow. More moderate antibody staining intensity was present in cells in the seminiferous ducts in testis, germinal center cells in the lymph node and tonsil and hematopoietic cells in the bone marrow. Low, but measureable presence of Cyclin B1 could be seen in cells in the endometrial stroma in endometrium, cells in the tubules in kidney, glandular cells in the cervix, uterine and endometrium, Langerhans in skin, lymphoid tissue in appendix, melanocytes in skin, squamous epithelial cells in the cervix and uterine. We were unable to detect Cyclin B1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Cyclin B1 expression as measured by anti-Cyclin B1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | ++ | ++ | ++ | ++ | ++ | + | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
| CCNB1 Variability | ++ | ++ | + | ++ | + | ++ | ++ | ++ | ++ | + | + | ++ | + | + | ++ | + | ++ | ++ | ++ | + |
Limitations and Warranty
enQuire Bio's Cyclin B1 Anti-Human, Mouse Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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