Western Blot of human Cerebellum Lysate using GFAP Monoclonal Antibody (GA-5).

Anti-GFAP Antibody Clone GA-5

$ 429.00
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SKU: 2670-MSM1
Species: Human, Mouse, Rat, Cow, Pig, Rabbit, and Chicken
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
2670-MSM1-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 2670-MSM1-P1
GFAP General Information
Alternate Names
GFAP, Glial fibrillary acidic protein
Molecular Weight
Chromosomal Location
Curated Database and Bioinformatic Data
Gene SymbolGFAP
Entrez Gene ID2670
Ensemble Gene IDENSG00000131095
RefSeq Protein Accession(s)NP_001124491, XP_016879940, NP_001229305, NP_002046
RefSeq mRNA Accession(s)XM_017024451, NM_002055 NM_001242376, NM_001131019
RefSeq Genomic Accession(s)NC_018928, NG_008401, NC_000017
UniProt ID(s)A7REI1, K7EMP8, P14136
UniGene ID(s)A7REI1, K7EMP8, P14136
HGNC ID(s)4235
Cosmic ID(s)GFAP
KEGG Gene ID(s)hsa:2670
PharmGKB ID(s)PA28647
General Description of GFAP.
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte, neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain, for documenting astrocytic differentiation in tumors outside the CNS.

Human, Mouse, Rat, Cow, Pig, Rabbit, and Chicken Anti-GFAP Antibody Product Attributes

Species: Human, Mouse, Rat, Cow, Pig, Rabbit, and Chicken
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-GFAP Antibody Clone: GA-5
Clone GA-5 Host and Isotype: Mouse IgG1
Anti-Human, Mouse, Rat, Cow, Pig, Rabbit, and Chicken GFAP Positive Control Sample: Brain or Astrocytoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: GFAP isolated from pig spinal cord
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

GFAP Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-GFAP antibody staining is expected to be primarily localized to the intermediate filaments. There is variability in either the signal strength or the localization of signal in intermediate filaments from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in GFAP antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of GFAP could be seen in. We were unable to detect GFAP in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's GFAP Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-GFAP Antibody Clone GA-5

https://enquirebio.com/wp-content/uploads/2019/01/GFAP IHC human Cerebellum (GA-5)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2019/01/GFAP Human Cerebellum Lysate Western.jpghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-2670-MSM1-P1-anti-GFAP-antibody-300x225.jpeg