Formalin-fixed, paraffin-embedded human Melanoma stained with MITF Monoclonal Antibody (D5 + MITF/915).
Formalin-fixed, paraffin-embedded human Melanoma stained with MITF Monoclonal Antibody (D5 + MITF/915).

Anti-Microphthalmia Transcription Factor Antibody Clone D5 + MITF/915

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 4286-MSM4
Species: Human, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 4286-MSM4-P1
Microphthalmia Transcription Factor General Information
Alternate Names
Microphthalmia-associated transcription factor class E basic helix-loop-helix protein 32, bHLHe32, MITF
Molecular Weight
52-56kDa (doublet)
Chromosomal Location
3p14.1
Curated Database and Bioinformatic Data
Gene SymbolMITF
Entrez Gene ID4286
Ensemble Gene IDENSG00000187098
RefSeq Protein Accession(s)XP_011532024, XP_016861935, NP_937801, NP_937820, XP_005264812, XP_016861936, NP_001341537, XP_005264811, XP_011532028, NP_001341534, XP_011532027, XP_016861937, NP_000239, NP_001171897, NP_001341533, NP_001341535, XP_011532025, NP_001171896, XP_016861933, XP_016861934, NP_001341536, NP_006713, XP_006713227, NP_937802, NP_937821
RefSeq mRNA Accession(s)NM_001354606, NM_198158, XM_017006447, NM_001354607, NM_006722, NM_198159, XM_006713164, XM_017006445, XM_017006446, NM_001184967, XM_011533722, NM_000248, NM_001354605, NM_198177, NM_001184968, XM_011533726, XM_017006448, XM_011533723, XM_011533725, XM_017006444, NM_001354608, XM_005264755, NM_001354604, NM_198178
RefSeq Genomic Accession(s)NC_000003, NC_018914, NG_011631,
UniProt ID(s)A0A087WXU1, B4DNC7, O75030, Q8WYR3
UniGene ID(s)A0A087WXU1, B4DNC7, O75030, Q8WYR3
HGNC ID(s)7105
Cosmic ID(s)MITF
KEGG Gene ID(s)hsa:4286
PharmGKB ID(s)PA30823
General Description of Microphthalmia Transcription Factor.
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development, survival of melanocytes, retinal pigment epithelium,, also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1, TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness, pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H,, MITF-M, which differ in the amino-terminal domain, in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary, metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi, dysplastic nevi.

Human, Mouse (-), and Rat (-) Anti-Microphthalmia Transcription Factor Antibody Product Attributes

Species: Human, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Microphthalmia Transcription Factor Antibody Clone: D5 + MITF/915
Clone D5 + MITF/915 Host and Isotype: Mouse IgG
Anti-Human, Mouse (-), and Rat (-) Microphthalmia Transcription Factor Positive Control Sample: Jurkat, A-431, HeLa or 501 Mel human melanoma cells or Melanoma
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: NH2 terminus fragment of human Mi protein (D5); Recombinant human MiTF protein (MITF/915)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Microphthalmia Transcription Factor Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in Microphthalmia Transcription Factor antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in melanocytes in skin. More moderate antibody staining intensity was present in melanocytes in skin. Low, but measureable presence of Microphthalmia Transcription Factor could be seen in cells in the tubules in kidney, germinal center cells in the tonsil, glandular cells in the epididymis and stomach, hematopoietic cells in the bone marrow, lymphoid tissue in appendix, macrophages in lung, non-germinal center cells in the lymph node and tonsil and smooth muscle cells in the smooth muscle. We were unable to detect Microphthalmia Transcription Factor in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Microphthalmia Transcription Factor expression as measured by anti-Microphthalmia Transcription Factor antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - - +++ - - - - - - - + -
MITF Variability + + + + + ++ + + + + ++ + + + + + + + ++ +

Limitations and Warranty

enQuire Bio's Microphthalmia Transcription Factor Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Microphthalmia Transcription Factor Antibody Clone D5 + MITF/915

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