Formalin-fixed, paraffin-embedded human Tonsil stained with MRP14 Monoclonal Antibody (MRP14/84)
Formalin-fixed, paraffin-embedded human Tonsil stained with MRP14 Monoclonal Antibody (MRP14/84)

Anti-Myeloid-Related Proteins 14 Antibody Clone MRP14/840

$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 6280-MSM3
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgM kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
6280-MSM3-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 6280-MSM3-P1
Myeloid-Related Proteins 14 General Information
Alternate Names
S100 calcium-binding protein A9, S100A9, migration inhibitory factor-related protein 14, MRP14, calgranulin B, S100A9
Molecular Weight
14kDa
Chromosomal Location
1q21
Curated Database and Bioinformatic Data
Gene SymbolS100A9
Entrez Gene ID6280
Ensemble Gene IDENSG00000163220
RefSeq Protein Accession(s)NP_002956
RefSeq mRNA Accession(s)NM_002965
RefSeq Genomic Accession(s)NC_018912, NC_000001
UniProt ID(s)P06702
UniGene ID(s)P06702
HGNC ID(s)10499
Cosmic ID(s)S100A9
KEGG Gene ID(s)hsa:6280
PharmGKB ID(s)PA34911
General Description of Myeloid-Related Proteins 14.
Recognizes a protein of14kDa, identified as MRP-14 (also known as Calgranulin B or S100AA9). It comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes, is also found in monocytes, macrophages, ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra-, intracellularly, neutrophils only intracellularly. It is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore, it is also detectable in feces. Elevated levels have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools,, synovial fluid during infection, inflammatory conditions. This MAb reacts with neutrophils, monocytes,, macrophages,, has been shown as an important marker for identifying macrophages in tissue sections.

Human Anti-Myeloid-Related Proteins 14 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed. Not suitable for frozen tissues.) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Myeloid-Related Proteins 14 Antibody Clone: MRP14/840
Clone MRP14/840 Host and Isotype: Mouse IgM kappa
Anti-Human Myeloid-Related Proteins 14 Positive Control Sample: Tonsil, lymph node, or spleen
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human MRP14 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Myeloid-Related Proteins 14 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-S100A9 antibody staining is expected to be primarily localized to the cell junctions and cytosol.

Observed Antibody Staining Data By Tissue Type:

Variations in Myeloid-Related Proteins 14 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hematopoietic cells in the bone marrow, squamous epithelial cells in the cervix, uterine, esophagus and oral mucosa, epidermal cells in the skin, cells in the red pulp in spleen and squamous epithelial cells in the tonsil and vagina. More moderate antibody staining intensity was present in hematopoietic cells in the bone marrow, squamous epithelial cells in the cervix, uterine, esophagus and oral mucosa, epidermal cells in the skin, cells in the red pulp in spleen and squamous epithelial cells in the tonsil and vagina. Low, but measureable presence of Myeloid-Related Proteins 14 could be seen in cells in the white pulp in spleen. We were unable to detect Myeloid-Related Proteins 14 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Myeloid-Related Proteins 14 expression as measured by anti-Myeloid-Related Proteins 14 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - + - + - + - ++ - - - - - - ++ - - - -
S100A9 Variability ++ + ++ + ++ + ++ ++ ++ ++ + ++ ++ ++ + ++ + + + ++

Limitations and Warranty

enQuire Bio's Myeloid-Related Proteins 14 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Myeloid-Related Proteins 14 Antibody Clone MRP14/840

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