Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NF421).
Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NF421).

Anti-Neurofilament Antibody Clone NF421

$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 4744-MSM1
Species: Human, Mouse, Rat, Pig, and Chicken
Tested Applications: Flow Cytometry, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
4744-MSM1-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 4744-MSM1-P1
Neurofilament General Information
Alternate Names
NEFH, Neurofilament H, NF2, anti-NEFH antibody, anti-Neurofilament H antibody, anti-NF2 antibody
Molecular Weight
Chromosomal Location
Curated Database and Bioinformatic Data
Gene SymbolNEFH
Entrez Gene ID4744
Ensemble Gene IDENSG00000100285
RefSeq Protein Accession(s)XP_011528502, NP_066554
RefSeq mRNA Accession(s)NM_021076, XM_011530200
RefSeq Genomic Accession(s)NG_008404, NC_018933, NC_000022
UniProt ID(s)P12036
UniGene ID(s)P12036
HGNC ID(s)7737
Cosmic ID(s)NEFH
KEGG Gene ID(s)hsa:4744
PharmGKB ID(s)PA31540
General Description of Neurofilament.
This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons, dendrites, are found in neurons, peripheral nerves,, sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M), 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine,, endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas,, neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal, extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin,, oat cell carcinomas of the lung also express neurofilament.

Human, Mouse, Rat, Pig, and Chicken Anti-Neurofilament Antibody Product Attributes

Species: Human, Mouse, Rat, Pig, and Chicken
Tested Applications: Flow Cytometry, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Neurofilament Antibody Clone: NF421
Clone NF421 Host and Isotype: Mouse IgG1
Anti-Human, Mouse, Rat, Pig, and Chicken Neurofilament Positive Control Sample: Brain, Neuroblastoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human recombinant neurofilament protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Neurofilament Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-NEFH antibody staining is expected to be primarily localized to the centrosome, nuclear bodies and nucleus.

Observed Antibody Staining Data By Tissue Type:

Variations in Neurofilament antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in peripheral nerve/ganglion in colon and peripheral nerve in mesenchymal tissue. More moderate antibody staining intensity was present in peripheral nerve/ganglion in colon and peripheral nerve in mesenchymal tissue. Low, but measureable presence of Neurofilament could be seen in. We were unable to detect Neurofilament in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Neurofilament expression as measured by anti-Neurofilament antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - + - - - - - - - - - - - - -
NEFH Variability + + + + + + ++ + + + + + + + + + + + + +

Limitations and Warranty

enQuire Bio's Neurofilament Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Neurofilament Antibody Clone NF421