Immunohistochemical staining of Perforin  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Perforin [5B10] Antibody (cGMP).

$ 135.00$ 725.00
Please Select Product Options Below To View The Catalog Number.

Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1

Product NumberDescriptionPrice
QIVD182-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD182-0.5ml Size: 0.5 ml, Format: Concentrate $ 435.00
QIVD182-1ml Size: 1 ml, Format: Concentrate $ 725.00
Shipping Information
In Stock
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:


Bioactivity (test results eg. IU/ml):

SKU: QIVD182-0.1ml
Perforin General Information
Alternate Names
Molecular Weight
61.4 kDa
Chromosomal Location
q22.1 [chr: 10] [chr_start: 70597348] [chr_end: 70602759] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolPRF1
Entrez Gene ID5551
RefSeq Protein Accession(s)NP_001076585; NP_005032
RefSeq mRNA Accession(s)NM_001083116; NM_005041;
RefSeq Genomic Accession(s)NG_009615; NC_000010
UniProt ID(s)P14222
PharmGKB ID(s)PA33732
KEGG Gene ID(s)hsa:5551
Associated Diseases (KEGG IDs)Familial hemophagocytic lymphohistiocytosis 2 (FHL2) [MIM:603553]: A rare disorder characterized by immune dysregulation with hypercytokinemia, defective function of natural killer cell, and massive infiltration of several organs by activated lymphocytes and macrophages. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, and less frequently neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits and ataxia. {ECO:0000269|PubMed:10583959, ECO:0000269|PubMed:11179007}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of Perforin .
This antibody is specific to a 70 kDa protein, which is a potent cytolytic pore-forming protein. Perforin is a specific marker of functionally active cytotoxic T-lymphocytes and natural killer cells. Perforin mediates cytolysis of target cells by membrane damage and apoptosis.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:10-1:20
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Perforin Antibody Clone: 5B10
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Spleen
Cellular Localization of Antibody 5B10 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Recombinant protein corresponding to an external region of the C-terminus of the perforin molecule
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Perforin Information for Pathologists


Perforin is the pore-forming protein that drives osmotic lysis of target cells enables granzymes to enter the target cells and activate apoptosis. The expression of perforin is primarily upregulated in activated cytotoxic cells such as CD8+ T-cells and NK cells but is also found in some populations of CD4+ T-cells.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

There are no reviews yet.

Be the first to review “Perforin [5B10] Antibody (cGMP).”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title




  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Chat: See lower right corner. | | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

Perforin [5B10] Antibody (cGMP).