Human and Mouse Anti-Plakophilin-1 Antibody Product Attributes
Species: Human and Mouse
Tested Applications: Western Blot.
Clonality: Monoclonal
Anti-Plakophilin-1 Antibody Clone: 10B2
Clone 10B2 Host and Isotype: Mouse IgG1
Anti-Human and Mouse Plakophilin-1 Positive Control Sample: HaCat Cells.
Cellular Localization of Antibody <10B2 Staining: Cell Surface and nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: N-terminus of human Plakophilin-1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Plakophilin-1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-pkp1 antibody staining is expected to be primarily localized to the nucleoplasm.Observed Antibody Staining Data By Tissue Type:
Variations in Plakophilin-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in squamous epithelial cells in the esophagus, keratinocytes in skin, Langerhans in skin and melanocytes in skin. More moderate antibody staining intensity was present in squamous epithelial cells in the esophagus, keratinocytes in skin, Langerhans in skin and melanocytes in skin. Low, but measureable presence of Plakophilin-1 could be seen in cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, endothelial cells in the cerebral cortex and colon, glandular cells in the fallopian tube, glial cells in the hippocampus, bile duct cells in the liver, myocytes in skeletal muscle, fibroblasts in skin, cells in the red pulp in spleen, cells in the white pulp in spleen and cells in the seminiferous ducts in testis. We were unable to detect Plakophilin-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Plakophilin-1 expression as measured by anti-Plakophilin-1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | ++ | + | +++ | ++ | ++ | + | ++ | ++ | ++ | ++ | + | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | +++ |
| PKP1 Variability | + | ++ | ++ | ++ | + | ++ | ++ | + | ++ | + | ++ | + | + | ++ | ++ | ++ | + | ++ | ++ | ++ |
Limitations and Warranty
enQuire Bio's Plakophilin-1 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
There are no reviews yet.