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Anti-Plakophilin-1 Antibody Clone 10B2

$ 118.00$ 418.00
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SKU: 5317-MSM1
Species: Human and Mouse
Tested Applications: Western Blot
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
5317-MSM1-P0 Size: 20 ug, Tag: Unconjugated $ 118.00
5317-MSM1-P0-P1 Size: 100 ug, Tag: Unconjugated $ 298.00
5317-MSM1-P0-P2 Size: 200 ug, Tag: Unconjugated $ 418.00
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In Stock.
Flat Rate Shipping Anywhere in the US: $45
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SKU: 5317-MSM1-P0
Plakophilin-1 General Information
Alternate Names
Plakophilin-1, PKP1
Molecular Weight
75kDa
Chromosomal Location
1q32
Curated Database and Bioinformatic Data
Gene SymbolPKP1
Entrez Gene ID5317
Ensemble Gene IDENSG00000081277
RefSeq Protein Accession(s)NP_000290, NP_001005337
RefSeq mRNA Accession(s)NM_000299, NM_001005337
RefSeq Genomic Accession(s)NC_018912, NG_023337, NC_000001
UniProt ID(s)Q13835, A0A024R952
UniGene ID(s)Q13835, A0A024R952
HGNC ID(s)9023
Cosmic ID(s)PKP1
KEGG Gene ID(s)hsa:5317
PharmGKB ID(s)PA33356
General Description of Plakophilin-1.
Recognizes a protein of 75kDa, identified as Plakophilin-1. Its epitope maps between aa 1-27 human Plakophilin-1. Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.

Human and Mouse Anti-Plakophilin-1 Antibody Product Attributes

Species: Human and Mouse
Tested Applications: Western Blot.
Clonality: Monoclonal
Anti-Plakophilin-1 Antibody Clone: 10B2
Clone 10B2 Host and Isotype: Mouse IgG1
Anti-Human and Mouse Plakophilin-1 Positive Control Sample: HaCat Cells.
Cellular Localization of Antibody Cell Surface and nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: N-terminus of human Plakophilin-1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Plakophilin-1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-pkp1 antibody staining is expected to be primarily localized to the nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in Plakophilin-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in squamous epithelial cells in the esophagus, keratinocytes in skin, Langerhans in skin and melanocytes in skin. More moderate antibody staining intensity was present in squamous epithelial cells in the esophagus, keratinocytes in skin, Langerhans in skin and melanocytes in skin. Low, but measureable presence of Plakophilin-1 could be seen in cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, endothelial cells in the cerebral cortex and colon, glandular cells in the fallopian tube, glial cells in the hippocampus, bile duct cells in the liver, myocytes in skeletal muscle, fibroblasts in skin, cells in the red pulp in spleen, cells in the white pulp in spleen and cells in the seminiferous ducts in testis. We were unable to detect Plakophilin-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Plakophilin-1 expression as measured by anti-Plakophilin-1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ + +++ ++ ++ + ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ ++ ++ +++
PKP1 Variability + ++ ++ ++ + ++ ++ + ++ + ++ + + ++ ++ ++ + ++ ++ ++

Limitations and Warranty

enQuire Bio's Plakophilin-1 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Plakophilin-1 Antibody Clone 10B2