FFPE human rhabdomyosarcoma sections stained with 100 ul anti-Myogenin (clone MYOG/2660) at 1:100. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Data from SDS-PAGE analysis of Anti-Myogenin antibody (Clone MYOG/2660). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.Analysis of Protein Array containing more than 19,000 full-length human proteins using Myogenin Mouse Monoclonal Antibody (MYOG/2660). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Myogenin (Skeletal Muscle Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 228.00$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 4656-MSM4
Species: Human
Tested Applications: IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG1, kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
4656-MSM4-P0 Size: 20 ug, Tag: Unconjugated $ 199.00
4656-MSM4-P1 Size: 100 ug, Tag: Unconjugated $ 429.00
4656-MSM4-P1ABX Size: 100 ug, Tag: Unconjugated $ 429.00
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SKU: 4656-MSM4-P0
Myogenin (Skeletal Muscle Marker) General Information
Alternate Names
bHLHc3, cb553, Class C basic helix-loop-helix protein 3, Myf-4, MYF4, MYOG, Myogenic factor 4
Molecular Weight
34kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol4656
Entrez Gene IDMYOG
UniProt ID(s)P15173
UniGene ID(s)Hs2830
COSMIC ID Link(s)MYOG
KEGG Gene ID(s)hsa:4656
General Description of Myogenin (Skeletal Muscle Marker).
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.

Human Anti-Myogenin (Skeletal Muscle Marker) Antibody Product Attributes

Species: Human
Tested Applications: IHC.
Clonality: Monoclonal
Anti-Myogenin (Skeletal Muscle Marker) Antibody Clone: MYOG/2660
Clone MYOG/2660 Host and Isotype: Mouse IgG1, kappa
Anti-Human Myogenin (Skeletal Muscle Marker) Positive Control Sample: Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.
Cellular Localization of Antibody MYOG/2660 Staining: Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant full-length human myogenin (MYOG) protein
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

Myogenin (Skeletal Muscle Marker) Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-MYOG antibody staining is expected to be primarily localized to the nucleoplasm. There is variability in either the signal strength or the localization of signal in nucleoplasm from cell to cell.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Myogenin expression as measured by anti-Myogenin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + + - - - + + + - + + ++ - + + - - + - -
MYOG Variability ++ ++ ++ + ++ ++ ++ ++ + ++ ++ ++ ++ ++ +++ ++ ++ ++ ++ ++

Limitations and Warranty

enQuire Bio's product, Myogenin (Skeletal Muscle Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Tested against >20,000 Human Proteins

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Myogenin (Skeletal Muscle Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

https://enquirebio.com/wp-content/uploads/2019/01/Anti-Myogenin Specificity Assay (Array)-300x120.jpghttps://enquirebio.com/wp-content/uploads/2019/01/Myogenin IHC human Rhabdomyosarcoma (MYOG 2660)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2019/01/Anti-Myogenin(MYOG 2660) Gel-231x300.jpghttps://enquirebio.com/wp-content/uploads/2018/06/enQuire-Bio-4656-MSM4-P0-anti-Myogenin-Skeletal-Muscle-Marker-antibody-1-300x120.jpeg