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Recombinant Bovine aFGF Protein

$ 89.00$ 1,880.00
Please Select A Product To View The SKU.

SKU: QP10508
Species: Bovine
Applications: Bioactive
Available Tags: Untagged
Available Hosts: Native Protein
Purity: Greater than 90%.
Length (Amino Acids): 155

Size
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Shipping Information
1-3 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP10508-1ug
Recombinant Bovine aFGF General Information
aFGF  protein 3D structural model from Catalog of Somatic Mutations in Cancer originally published in the paper COSMIC: somatic cancer genetics at high-resolution
Alternate Names
HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a.
Molecular Weight
17.5 kDa
Chromosomal Location
On chromosome 7
Curated Database and Bioinformatic Data
Gene SymbolFGF1
Entrez Gene ID281160
UniProt ID(s)P03968
COSMIC ID Link(s)FGF1
KEGG Gene ID(s)bta:281160
General Description of Recombinant Bovine aFGF.
Fibroblast Growth Factor-acidic Bovine (FGF-1) purified from Bovine Brain contains a 17 kDa and a 20 kDa polypeptide chain. The 17 kDa peptide is derived from the 20K peptide by restricted proteolysis. (See Jaye et al²). The FGF acidic is purified by using an optimized multi-step FPLC method for maximum separation from contaminants.

Bovine aFGF Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant aFGF based upon sequence from Bovine
Host: QP10508 protein expressed in Native Protein.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of aFGF was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized aFGF in sterile 50mM Na2HPO4 pH 7, and 0.5% albumin. The Recommended concentration in cell culture: 1-20ng/ml.
Bioactivity Data: Stimulates growth of bovine capillary endothelial cells by 3-5 fold over 5% calf serum at 10-25ng/ml FGF and 0ug/ml heparin.
Amino Acid Sequence: MAEGETTTFT ALTEKFNLPL GNYKKPKLLY CSNGGYFLRI LPDGTVDGTK DRSDQHIQLQ LCAESIGEVY IKSTETGQFL AMDTDGLLYG SQTPNEECLF LERLEENHYN TYISKKHAEK HWFVGLKKNG RSKLGPRTHF GQKAILFLPL PVSSD
Purity: Greater than 90%.
Endotoxin Levels:
Buffer: Each 5µg aFGF were lyophilized from 0.5ml solution containing 1mM sodium phosphate, pH 7 after filtration over a low binding membrane.
Storage Conditions: Lyophilized aFGF although stable at room temperature for 2 weeks, should be stored desiccated below -18°C. Upon reconstitution aFGF should be stored at 4°C between 2-3 weeks and for future use below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Bovine aFGF Protein